Rat Recombinant FSTL1 protein, His Tag (V2LY-0526-LY9383)

Name: Rat Recombinant FSTL1 protein, His Tag
SKU: V2LY-0526-LY9383
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Rat

Tag

His Tag

Protein Construction

This product is Rat Recombinant FSTL1 protein, His Tag consist of Amino Acid: 1-306 and predicts a molecular mass of 34 kDa.

Molecule Mass

34 kDa

Sequence

Amino Acid: 1-306

Species

Rat

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Follistatin Like 1

Function

Secreted glycoprotein that is involved in various physiological processes, such as angiogenesis, regulation of the immune response, cell proliferation and differentiation (PubMed:29212066, PubMed:22265692).

Plays a role in the development of the central nervous system, skeletal system, lungs, and ureter (By similarity).

Promotes endothelial cell survival, migration and differentiation into network structures in an AKT-dependent manner. Also promotes survival of cardiac myocytes (By similarity).

Initiates various signaling cascades by activating different receptors on the cell surface such as DIP2A, TLR4 or BMP receptors (PubMed:20054002, PubMed:22265692).

Biological Process

Cell differentiation Source: GO_Central
Endothelial cell differentiation Source: UniProtKB
Endothelial cell migration Source: UniProtKB
Hematopoietic stem cell homeostasis Source: Ensembl
Negative regulation of apoptotic process Source: UniProtKB
Regulation of BMP signaling pathway Source: GO_Central

Cellular Location

Secreted

Ogiwara, Y., Nakagawa, M., Nakatani, F., Uemura, Y., Zhang, R., & Kudo-Saito, C. (2022). Blocking FSTL1 boosts NK immunity in treatment of osteosarcoma. Cancer Letters, 537, 215690.

Rao, J., Wang, H., Ni, M., Wang, Z., Wang, Z., Wei, S., ... & Lu, L. (2022). FSTL1 promotes liver fibrosis by reprogramming macrophage function through modulating the intracellular function of PKM2. Gut, 71(12), 2539-2550.

Li, X., Fang, Y., Jiang, D., Dong, Y., Liu, Y., Zhang, S., ... & Ning, W. (2021). Targeting FSTL1 for multiple fibrotic and systemic autoimmune diseases. Molecular Therapy, 29(1), 347-364.

Loh, J. J., Li, T. W., Zhou, L., Wong, T. L., Liu, X., Ma, V. W., ... & Ma, S. (2021). FSTL1 Secreted by Activated Fibroblasts Promotes Hepatocellular Carcinoma Metastasis and StemnessFSTL1 from Activated Fibroblasts on HCC. Cancer research, 81(22), 5692-5705.

Li, W., Alahdal, M., Deng, Z., Liu, J., Zhao, Z., Cheng, X., ... & Zhang, J. (2020). Molecular functions of FSTL1 in the osteoarthritis. International immunopharmacology, 83, 106465.

Nie, E., Miao, F., Jin, X., Wu, W., Zhou, X., Zeng, A., ... & You, Y. (2019). Fstl1/DIP2A/MGMT signaling pathway plays important roles in temozolomide resistance in glioblastoma. Oncogene, 38(15), 2706-2721.

Li, X., Li, L., Chang, Y., Ning, W., & Liu, X. (2019). Structural and functional study of FK domain of Fstl1. Protein Science, 28(10), 1819-1829.

Gu, C., Wang, X., Long, T., Wang, X., Zhong, Y., Ma, Y., ... & Li, Z. (2018). FSTL1 interacts with VIM and promotes colorectal cancer metastasis via activating the focal adhesion signalling pathway. Cell death & disease, 9(6), 654.

Kudo-Saito, C., Ishida, A., Shouya, Y., Teramoto, K., Igarashi, T., Kon, R., ... & Toyoura, M. (2018). Blocking the FSTL1-DIP2A axis improves anti-tumor immunity. Cell reports, 24(7), 1790-1801.

Ni, X., Cao, X., Wu, Y., & Wu, J. (2018). FSTL1 suppresses tumor cell proliferation, invasion and survival in non-small cell lung cancer. Oncology Reports, 39(1), 13-20.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Recombinant FSTL1 protein, hFc Tag (CAT#: V2LY-0526-LY8338)

Mouse Recombinant FSTL1 protein, His Tag (CAT#: V2LY-0526-LY8339)

Human Recombinant FSTL1 protein, His Tag (CAT#: V2LY-0526-LY4188)

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Mouse Recombinant FSTL1 protein, hFc Tag (CAT#: V2LY-0526-LY8338)

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Rat Anti-FSTL1 Recombinant Antibody (CBXF-2679) (CAT#: V2LY-1225-LY175)

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Mouse Recombinant FSTL1 protein, His Tag (CAT#: V2LY-0526-LY8339)

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Rat Anti-FSTL1 Recombinant Antibody (CBXF-2680) (CAT#: V2LY-1225-LY176)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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