Rat Recombinant SPARC protein, His Tag (V2LY-0526-LY9595)

Name: Rat Recombinant SPARC protein, His Tag
SKU: V2LY-0526-LY9595
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Rat

Tag

His Tag

Protein Construction

This product is Rat Recombinant SPARC protein, His Tag consist of Amino Acid: 1-301 and predicts a molecular mass of 33.8 kDa.

Molecule Mass

33.8 kDa

Verified

HPLC

Sequence

Amino Acid: 1-301

Species

Rat

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

SPARC

Function

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.

Biological Process

Biological Process anatomical structure developmentManual Assertion Based On ExperimentIBA:GO_Central
Biological Process negative regulation of angiogenesisManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of endothelial cell proliferationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of endothelial cell migrationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of cell morphogenesisManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Secreted, extracellular space, extracellular matrix, basement membrane
In or around the basement membrane.

Involvement in disease

Osteogenesis imperfecta 17 (OI17):
An autosomal recessive form of osteogenesis imperfecta, a connective tissue disorder characterized by low bone mass, bone fragility and susceptibility to fractures after minimal trauma. Disease severity ranges from very mild forms without fractures to intrauterine fractures and perinatal lethality. Extraskeletal manifestations, which affect a variable number of patients, are dentinogenesis imperfecta, hearing loss, and blue sclerae.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant SPARC protein, His Tag (CAT#: V2LY-0526-LY6922)

Mouse Recombinant SPARC protein, His Tag (CAT#: V2LY-0526-LY8927)

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Mouse Recombinant SPARC protein, His Tag (CAT#: V2LY-0526-LY8927)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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