Rat Ucp1 ELISA Kit (V2LY-0626-LY2436)
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Basic Information
- Pre-coated ELISA Plate: 12 wells * 8 detachable strips
- Standard solution: 0.5ml x1
- Standard diluent: 3ml x1
- Streptavidin-HRP: 6ml x1
- Stop solution: 6ml x1
- Substrate solution A: 6ml x1
- Substrate solution B: 6ml x1
- Wash buffer concentrate (25x): 20ml x1
- Biotinylated antibody: 1ml x1
Formulations & Storage [For reference only, actual COA shall prevail!]
Target
Functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane (PubMed:24196960).
However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs. Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP. Regulates the production of reactive oxygen species/ROS by mitochondria (By similarity).
Biological Process brown fat cell differentiation Source:HGNC-UCL
Biological Process cellular response to cold Source:Ensembl
Biological Process cellular response to dehydroepiandrosterone Source:Ensembl
Biological Process cellular response to fatty acid Source:UniProtKB
Biological Process cellular response to hormone stimulus Source:UniProtKB
Biological Process cellular response to reactive oxygen species Source:UniProtKB
Biological Process diet induced thermogenesis Source:Ensembl
Biological Process mitochondrial transmembrane transport Source:UniProtKB
Biological Process mitochondrial transport Source:Ensembl
Biological Process positive regulation of cold-induced thermogenesis Source:YuBioLabBy Similarity
Biological Process proton transmembrane transport Source:UniProtKB1 Publication
Biological Process regulation of reactive oxygen species biosynthetic process Source:UniProtKB
Biological Process regulation of transcription by RNA polymerase II Source:Ensembl
Biological Process response to cold Source:GO_Central1 Publication
Biological Process response to nutrient levels Source:UniProtKB
Biological Process response to temperature stimulus Source:UniProtKB
Helical: 11-32
Mitochondrial matrix: 33-73
Helical: 74-96
Mitochondrial intermembrane: 97-116
Helical: 117-133
Mitochondrial matrix: 134-178
Helical: 179-195
Mitochondrial intermembrane: 196-212
Helical: 213-232
Mitochondrial matrix: 233-266
Helical: 267-289
Mitochondrial intermembrane: 290-307
May undergo ubiquitin-mediated proteasomal degradation.
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Please try the standard protocols which include: protocols, troubleshooting and guide.
Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols
Alternative Versions
Mouse Ucp1 ELISA Kit (CAT#: V2LY-0626-LY2673)
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Custom Antibody Labeling
We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).
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