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Human Receptor Antibody Array (Glass Slide, 40 Targets) (Y0103) (AbAr-0103-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Human Receptor Antibody Array detects 40 Human Soluble Receptors. It is suitable for all liquid sample types.
See all Receptor antibodies

Specifications

Target Array
Receptor Array
Target
4-1BB (TNFRSF9/CD137), CD30 (TNFRSF8), Endoglin (CD105), GITR (TNFRSF18), IL-2 R gamma (Common gamma Chain), Lipocalin-2 (NGAL), NRG1-beta 1 (HRG1-beta 1), TRAIL R3 (TNFRSF10C), ALCAM (CD166), CD40 Ligand (TNFSF5), ErbB3, HVEM (TNFRSF14), IL-10 R beta, L-Selectin (CD62L), PDGF R beta, Trappin-2, CD80 (B7-1), CEACAM-1, E-Selectin, ICAM-3 (CD50), IL-17 RA, LYVE-1, PECAM-1, uPAR, BCMA, DR6 (TNFRSF21), Fas (TNFRSF6/Apo-1), Contactin-2 (TAG1), IL-21 R, MICA, RAGE, VCAM-1 (CD106), CD14, Dtk, Flt-3 Ligand, IL-1 R1, LIMPII, MICB, TIM-1 (KIM-1), XEDAR
Species Reactivity
Human
Sample Type
Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates, Other Body Fluids

Applications

Application
Multiplex Protein Detection
Assay Type
Quantitative
Solid Support
Glass Slide
Detection Assay
Sandwich-based
Protocol Outline
Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis
Components
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction
In biochemistry and pharmacology, receptors are chemical structures, composed of protein, that receive and transduce signals that may be integrated into biological systems. These signals are typically chemical messengers, which bind to a receptor, they cause some form of cellular/tissue response, e.g. a change in the electrical activity of a cell. There are three main ways the action of the receptor can be classified: relay of signal, amplification, or integration. Relaying sends the signal onward, amplification increases the effect of a single ligand, and integration allows the signal to be incorporated into another biochemical pathway. In this sense, a receptor is a protein-molecule that recognizes and responds to endogenous chemical signals. For example, an acetylcholine receptor recognizes and responds to its endogenous ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug targets, such as enzymes, transporters, and ion channels.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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