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Human Receptor Antibody Array (Glass Slide, 40 Targets) (Y0567) (AbAr-0567-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The product provided herein is a Human Receptor Antibody Array, which can be used for semi-quantitative detection of 40 human cytokines. It is suitable for a range of liquid forms including Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates.
See all Receptor antibodies

Specifications

Target Array
Receptor Array
Target
4-1BB(TNFRSF9/CD137), ALCAM(CD166), CD80(B7-1), BCMA, CD14, CD30(TNFRSF8), CD40 Ligand(TNFSF5), CEACAM-1, DR6(TNFRSF21), Dtk, Endoglin(CD105), ErbB3, E-Selectin, Fas(TNFRSF6/Apo-1), Flt-3 Ligand, GITR(TNFRSF18), HVEM(TNFRSF14), ICAM-3(CD50), Contactin-2(TAG1), IL-1 R1, IL-2 R gamma(Common gamma Chain), IL-10 R beta, IL-17 RA, IL-21 R, LIMPII, Lipocalin-2(NGAL), L-Selectin(CD62L), LYVE-1, MICA, MICB, NRG1-beta 1(HRG1-beta 1), PDGF R beta, PECAM-1, RAGE, TIM-1(KIM-1), TRAIL R3(TNFRSF10C), Trappin-2, uPAR, VCAM-1(CD106), XEDAR
Species Reactivity
Human
Sample Type
Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates

Applications

Application
Multiplexed Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Glass Slide
Detection Assay
Sandwich-based Assay
Protocol Outline
Dry the array glass slide
Block glass slide surface
Incubate with samples
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Fluor Conjugated Streptavidin
Scan with an antibody array laser scanner
Analyze data
Components
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Washing Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at -20°C for 6 months. Once thawed, please keep reagents under suitable conditions respectively for no more than 3 months. Do not use past expiration date.

Background

Introduction
In biochemistry and pharmacology, receptors are chemical structures, composed of protein, that receive and transduce signals that may be integrated into biological systems. These signals are typically chemical messengers, which bind to a receptor, they cause some form of cellular/tissue response, e.g. a change in the electrical activity of a cell. There are three main ways the action of the receptor can be classified: relay of signal, amplification, or integration. Relaying sends the signal onward, amplification increases the effect of a single ligand, and integration allows the signal to be incorporated into another biochemical pathway. In this sense, a receptor is a protein-molecule that recognizes and responds to endogenous chemical signals. For example, an acetylcholine receptor recognizes and responds to its endogenous ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug targets, such as enzymes, transporters, and ion channels.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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