Rhesus Recombinant APLI protein, His Tag (V2LY-0526-LY9646)

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host
HEK293 Cells
Protein Species
Rhesus
Tag
His Tag
Protein Construction
This product is Rhesus Recombinant APLI protein, His Tag consist of Amino Acid: 1-503 and predicts a molecular mass of 53.86 kDa.
Molecule Mass
53.86 kDa
Verified
HPLC
Sequence
Amino Acid: 1-503
Species
Rhesus

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
≥95% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.
Endotoxin
Please contact us for more information.
Format
Lyophilized
Reconstitution
Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.
Buffer
Lyophilized from sterile PBS
Preservative
None
Storage
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
More Infomation

Target

Full Name
Alkaline Phosphatase, Intestinal
Biological Process
Dephosphorylation Source: UniProtKB
Digestion Source: Reactome
Phosphatidic acid biosynthetic process Source: Reactome
Cellular Location
Cell membrane

Lallès, J. P. (2020). Intestinal alkaline phosphatase in the gastrointestinal tract of fish: biology, ontogeny, and environmental and nutritional modulation. Reviews in Aquaculture, 12(2), 555-581.

Jiang, Y., Li, X., & Walt, D. R. (2020). Single‐molecule analysis determines isozymes of human alkaline phosphatase in serum. Angewandte Chemie International Edition, 59(41), 18010-18015.

Plaeke, P., De Man, J. G., Smet, A., Malhotra‐Kumar, S., Pintelon, I., Timmermans, J. P., ... & De Winter, B. Y. (2020). Effects of intestinal alkaline phosphatase on intestinal barrier function in a cecal ligation and puncture (CLP)‐induced mouse model for sepsis. Neurogastroenterology & Motility, 32(3), e13754.

Takeuchi, A., Hisamatsu, K., Okumura, N., Sugimitsu, Y., Yanase, E., Ueno, Y., & Nagaoka, S. (2020). IIAEK targets intestinal alkaline phosphatase (IAP) to improve cholesterol metabolism with a specific activation of IAP and downregulation of abca1. Nutrients, 12(9), 2859.

Lallès, J. P. (2019). Recent advances in intestinal alkaline phosphatase, inflammation, and nutrition. Nutrition reviews, 77(10), 710-724.

Ishige, T., Itoga, S., Utsuno, E., Nishimura, M., Yoshikawa, M., Kato, N., ... & Nomura, F. (2018). Variant in C-terminal region of intestinal alkaline phosphatase associated with benign familial hyperphosphatasaemia. Journal of medical genetics, 55(10), 701-704.

Parlato, M., Charbit‐Henrion, F., Pan, J., Romano, C., Duclaux‐Loras, R., Le Du, M. H., ... & Cerf‐Bensussan, N. (2018). Human ALPI deficiency causes inflammatory bowel disease and highlights a key mechanism of gut homeostasis. EMBO molecular medicine, 10(4), e8483.

Noda, S., Yamada, A., Nakaoka, K., & Goseki-Sone, M. (2017). 1-alpha, 25-Dihydroxyvitamin D3 up-regulates the expression of 2 types of human intestinal alkaline phosphatase alternative splicing variants in Caco-2 cells and may be an important regulator of their expression in gut homeostasis. Nutrition Research, 46, 59-67.

Wu, M., Wang, J., Wang, Z., Zhao, J., Hu, Y., & Chen, X. (2017). Sequence and functional analysis of intestinal alkaline phosphatase from Lateolabrax maculatus. Fish physiology and biochemistry, 43(6), 1463-1476.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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