Rhesus Recombinant CD1A & B2M protein, Hetero Dimer , His Tag (V2LY-0526-LY9665)

Name: Rhesus Recombinant CD1A & B2M protein, Hetero Dimer , His Tag
SKU: V2LY-0526-LY9665
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Rhesus

Tag

His Tag

Protein Construction

This product is Rhesus Recombinant CD1A & B2M protein, Hetero Dimer , His Tag consist of Amino Acid: 1-299, 1-119 and predicts a molecular mass of 45.2 kDa.

Molecule Mass

45.2 kDa

Polymeric

Hetero Dimer

Sequence

Amino Acid: 1-299, 1-119

Species

Rhesus

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD1a Molecule

Alternative Names

CD1a Molecule; T-Cell Surface Antigen T6/Leu-6; CD1A Antigen, A Polypeptide; HTa1 Thymocyte Antigen; CD1a Antigen; Epidermal Dendritic Cell Marker CD1a; Differentiation Antigen CD1-Alpha-3; T-Cell Surface Glycoprotein CD1a;

Function

Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.

Biological Process

Adaptive immune response Source: UniProtKB-KW
Antigen processing and presentation, endogenous lipid antigen via MHC class Ib Source: GO_Central
Antigen processing and presentation, exogenous lipid antigen via MHC class Ib Source: GO_Central
Immune response Source: UniProtKB
Positive regulation of T cell mediated cytotoxicity Source: GO_Central
Regulation of immune response Source: Reactome

Cellular Location

Endosome membrane; Cell membrane; Membrane raft. Subject to intracellular trafficking between the cell membrane and endosomes (PubMed:11231314). Localizes to cell surface lipid rafts (PubMed:18178838).

Topology

Extracellular: 17-300
Helical: 301-321
Cytoplasmic: 322-327

de Jong, A., & Ogg, G. (2021). CD1a function in human skin disease. Molecular Immunology, 130, 14-19.

Gapin, L. (2021). CD1a autoreactivity: When size does matter.

Yoo, H. J., Kim, N. Y., & Kim, J. H. (2021). Current Understanding of the Roles of CD1a-Restricted T Cells in the Immune System. Molecules and Cells, 44(5), 310.

Cotton, R. N., Wegrecki, M., Cheng, T. Y., Chen, Y. L., Veerapen, N., Le Nours, J., ... & Moody, D. B. (2021). CD1a selectively captures endogenous cellular lipids that broadly block T cell response. Journal of Experimental Medicine, 218(7), e20202699.

Cotton, R. N., Cheng, T. Y., Wegrecki, M., Le Nours, J., Orgill, D. P., Pomahac, B., ... & Moody, D. B. (2021). Human skin is colonized by T cells that recognize CD1a independently of lipid. The Journal of clinical investigation, 131(1).

Nicolai, S., Wegrecki, M., Cheng, T. Y., Bourgeois, E. A., Cotton, R. N., Mayfield, J. A., ... & de Jong, A. (2020). Human T cell response to CD1a and contact dermatitis allergens in botanical extracts and commercial skin care products. Science immunology, 5(43).

Sánchez-Martínez, D., Baroni, M. L., Gutierrez-Agüera, F., Roca-Ho, H., Blanch-Lombarte, O., González-García, S., ... & Menéndez, P. (2019). Fratricide-resistant CD1a-specific CAR T cells for the treatment of cortical T-cell acute lymphoblastic leukemia. Blood, The Journal of the American Society of Hematology, 133(21), 2291-2304.

Taheri, M., Danesh, H., Bizhani, F., Bahari, G., Naderi, M., & Hashemi, M. (2019). Association between genetic variants in CD1A and CD1D genes and pulmonary tuberculosis in an Iranian population. Biomedical reports, 10(4), 259-265.

Rahman, M. I., Jahan, I., Khalid, M. M., Jahan, I., Ahammad, R. U., Nahar, S., & Islam, Z. (2018). CD1A and CD1E gene polymorphisms are not associated with susceptibility to Guillain-Barré syndrome in the Bangladeshi population. Journal of neuroimmunology, 314, 8-12.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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