Rhesus Recombinant LAMP3 protein, His Tag (V2LY-0526-LY9763)

Name: Rhesus Recombinant LAMP3 protein, His Tag
SKU: V2LY-0526-LY9763
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Rhesus

Tag

His Tag

Protein Construction

This product is Rhesus Recombinant LAMP3 protein, His Tag consist of Amino Acid: 28-381 and predicts a molecular mass of 39 kDa.

Molecule Mass

39 kDa

Sequence

Amino Acid: 28-381

Species

Rhesus

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Lysosomal Associated Membrane Protein 3

Function

Lysosomal membrane glycoprotein which plays a role in the unfolded protein response (UPR) that contributes to protein degradation and cell survival during proteasomal dysfunction (PubMed:25681212).
Plays a role in the process of fusion of the lysosome with the autophagosome, thereby modulating the autophagic process (PubMed:24434718).
Promotes hepatocellular lipogenesis through activation of the PI3K/Akt pathway (PubMed:29056532).
May also play a role in dendritic cell function and in adaptive immunity (PubMed:9768752).
(Microbial infection) Plays a positive role in post-entry steps of influenza A virus replication, either virus uncoating, cytosolic transport, or nuclear import of viral components, and promotes nuclear accumulation of influenza nucleoprotein/NP at early stages of viral infection.
(Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F.
(Microbial infection) Promotes the intracellular proliferation of Salmonella typhimuium.

Biological Process

Adaptive immune responseIEA:UniProtKB-KW
Establishment of protein localization to organelleManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of cysteine-type endopeptidase activity involved in apoptotic processManual Assertion Based On ExperimentIMP:ParkinsonsUK-UCL
Negative regulation of proteasomal protein catabolic processManual Assertion Based On ExperimentIMP:ParkinsonsUK-UCL
Positive regulation of gene expressionManual Assertion Based On ExperimentIMP:ParkinsonsUK-UCL
Regulation of autophagyManual Assertion Based On ExperimentIMP:ParkinsonsUK-UCL
Regulation of viral life cycleManual Assertion Based On ExperimentIMP:ParkinsonsUK-UCL
Response to interferon-alphaManual Assertion Based On ExperimentIMP:ParkinsonsUK-UCL

Cellular Location

Cell surface
Lysosome membrane
Cytoplasmic vesicle membrane
Early endosome membrane
During dendritic cell maturation, detected on cytoplasmic vesicles (the MHC II compartment) that contain MHC II proteins, LAMP1, LAMP2 and LAMP3 (PubMed:9768752).
Detected on lysosomes in mature dendritic cells (PubMed:9768752).

Topology

Lumenal: 28-381
Helical: 382-402
Cytoplasmic: 403-416

Wang, Z., Wang, X., Jin, R., Liu, F., Rao, H., Wei, L., ... & Feng, B. (2023). LAMP3 expression in the liver is involved in T cell activation and adaptive immune regulation in hepatitis B virus infection. Frontiers in Immunology, 14, 1127572.

Tanaka, T., Warner, B. M., Michael, D. G., Nakamura, H., Odani, T., Yin, H., ... & Chiorini, J. A. (2022). LAMP3 inhibits autophagy and contributes to cell death by lysosomal membrane permeabilization. Autophagy, 18(7), 1629-1647.

Nakamura, H., Tanaka, T., Zheng, C., Afione, S. A., Warner, B. M., Noguchi, M., ... & Chiorini, J. A. (2022). Correction of LAMP3-associated salivary gland hypofunction by aquaporin gene therapy. Scientific Reports, 12(1), 18570.

Sun, Q., Wang, F., Chen, Q., Sarid, R., Li, X., & Kuang, E. (2022). Upregulation of ATF4-LAMP3 Axis by ORF45 Facilitates Lytic Replication of Kaposi’s Sarcoma-Associated Herpesvirus. Journal of Virology, 96(23), e01456-22.

Huang, F., Ma, G., Zhou, X., Zhu, X., Yu, X., Ding, F., ... & Liu, Z. (2020). Depletion of LAMP3 enhances PKA-mediated VASP phosphorylation to suppress invasion and metastasis in esophageal squamous cell carcinoma. Cancer Letters, 479, 100-111.

Tanaka, T., Warner, B. M., Odani, T., Ji, Y., Mo, Y. Q., Nakamura, H., ... & Chiorini, J. A. (2020). LAMP3 induces apoptosis and autoantigen release in Sjögren’s syndrome patients. Scientific Reports, 10(1), 15169.

Burton, T. D., Fedele, A. O., Xie, J., Sandeman, L. Y., & Proud, C. G. (2020). The gene for the lysosomal protein LAMP3 is a direct target of the transcription factor ATF4. Journal of Biological Chemistry, 295(21), 7418-7430.

Nishimura, J., Tanaka, H., Yamakoshi, Y., Hiramatsu, S., Tamura, T., Toyokawa, T., ... & Ohira, M. (2019). Impact of tumor-infiltrating LAMP-3 dendritic cells on the prognosis of esophageal squamous cell carcinoma. Esophagus, 16, 333-344.

Yang, C., Shen, S., Zheng, X., Ye, K., Sun, Y., Lu, Y., & Ge, H. (2019). Long noncoding RNA HAGLR acts as a microRNA‐143‐5p sponge to regulate epithelial‐mesenchymal transition and metastatic potential in esophageal cancer by regulating LAMP3. The FASEB Journal, 33(9), 10490-10504.

Liao, X., Song, L., Zhang, L., Wang, H., Tong, Q., Xu, J., ... & Zheng, H. (2018). LAMP3 regulates hepatic lipid metabolism through activating PI3K/Akt pathway. Molecular and cellular endocrinology, 470, 160-167.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant LAMP3 protein, His Tag (CAT#: V2LY-0526-LY5250)

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For research use only. Not intended for any clinical use.

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