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Mouse Anti-Skp1 Recombinant Antibody (4E11-F10-H1) (CBMAB-Z0171-LY)

The product is antibody recognizes Skp1. The antibody 4E11-F10-H1 immunoassay techniques such as: WB, IHC, ICC, FC.
See all Skp1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
4E11-F10-H1
Antibody Isotype
IgG1
Application
WB, IHC, ICC, FC

Basic Information

Immunogen
Purified recombinant fragment of human SKP1 expressed in E. Coli.
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
S-Phase Kinase Associated Protein 1
Entrez Gene ID
6500
UniProt ID
P63208
Alternative Names
OCP2; p19A; EMC19; SKP1A; OCP-II; TCEB1L; MGC34403
Function
Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5, CEP68 and probably NFKB2 (PubMed:25704143).
SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) directs ubiquitination of BCL6 and DTL but does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) directs ubiquitination of TTI1 and TELO2. SCF(FBXO10) directs ubiquitination of BCL2.
Biological Process
Biological Process histone H2A monoubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process maintenance of protein location in nucleusManual Assertion Based On ExperimentIPI:ParkinsonsUK-UCL
Biological Process positive regulation of ubiquitin protein ligase activityManual Assertion Based On ExperimentIDA:ParkinsonsUK-UCL
Biological Process protein K48-linked ubiquitinationIEA:Ensembl
Biological Process protein polyubiquitinationManual Assertion Based On ExperimentIDA:ParkinsonsUK-UCL
Biological Process protein ubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process SCF-dependent proteasomal ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
centrosome
Cul7-RING ubiquitin ligase complex
cytoplasm
cytosol
nucleoplasm
nucleus
SCF ubiquitin ligase complex
PTM
Undergoes autophagy-mediated degradation in the liver in a time-dependent manner.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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