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SKP1

This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7. [provided by RefSeq, Jul 2008]
Full Name
S-Phase Kinase Associated Protein 1
Function
Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5, CEP68 and probably NFKB2 (PubMed:25704143).
SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) directs ubiquitination of BCL6 and DTL but does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) directs ubiquitination of TTI1 and TELO2. SCF(FBXO10) directs ubiquitination of BCL2.
Biological Process
Biological Process histone H2A monoubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process maintenance of protein location in nucleusManual Assertion Based On ExperimentIPI:ParkinsonsUK-UCL
Biological Process positive regulation of ubiquitin protein ligase activityManual Assertion Based On ExperimentIDA:ParkinsonsUK-UCL
Biological Process protein K48-linked ubiquitinationIEA:Ensembl
Biological Process protein polyubiquitinationManual Assertion Based On ExperimentIDA:ParkinsonsUK-UCL
Biological Process protein ubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process SCF-dependent proteasomal ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
centrosome
Cul7-RING ubiquitin ligase complex
cytoplasm
cytosol
nucleoplasm
nucleus
SCF ubiquitin ligase complex
PTM
Undergoes autophagy-mediated degradation in the liver in a time-dependent manner.

Anti-SKP1 antibodies

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Target: SKP1
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human, Mouse, Rat, Monkey, Zebrafish
Clone: D3J4N
Application*: WB, IP
Target: SKP1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBXS-3901
Application*: E, IP, WB
Target: SKP1
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Human
Clone: CBXS-4242
Application*: E, IF, WB
Target: SKP1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBXS-2443
Application*: E, F, IH, WB
Target: SKP1
Host: Mouse
Specificity: Human
Clone: CBXS-2896
Application*: E, IF, WB
Target: SKP1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 3G222
Application*: E, IP, WB
Target: SKP1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human, Chicken, Dog, Frog, Mouse, Rat
Clone: 52/p19 Skp1
Application*: WB, IH, IP
Target: SKP1
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human, Monkey
Clone: CBWJC-4439
Application*: WB, IP, P, IF
Target: SKP1
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human, Mouse, Rat, Monkey, Zebrafish
Clone: CBWJC-4440
Application*: WB, IP
Target: Skp1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 4E11-F10-H1
Application*: WB, IH, IC, F
Target: SKP1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human, Mouse, Rat
Clone: CBXS-5033
Application*: F, IC, IF, IH, WB
Target: SKP1
Host: Mouse
Antibody Isotype: IgG
Specificity: Human
Clone: CBXS-0407
Application*: E, WB, P, F
Target: SKP1
Host: Rabbit
Antibody Isotype: IgG
Specificity: Mouse, Rat, Human
Clone: CBXS-1483
Application*: WB, P, F
Target: SKP1
Host: Rabbit
Antibody Isotype: IgG
Specificity: Mouse, Rat, Human
Clone: CBXS-1484
Application*: WB, F, IF
For Research Use Only. Not For Clinical Use.
(P): Predicted
* Abbreviations
IFImmunofluorescence
IHImmunohistochemistry
IPImmunoprecipitation
WBWestern Blot
EELISA
MMicroarray
CIChromatin Immunoprecipitation
FFlow Cytometry
FNFunction Assay
IDImmunodiffusion
RRadioimmunoassay
TCTissue Culture
GSGel Supershift
NNeutralization
BBlocking
AActivation
IInhibition
DDepletion
ESELISpot
DBDot Blot
MCMass Cytometry/CyTOF
CTCytotoxicity
SStimulation
AGAgonist
APApoptosis
IMImmunomicroscopy
BABioassay
CSCostimulation
EMElectron Microscopy
IEImmunoelectrophoresis
PAPeptide Array
ICImmunocytochemistry
PEPeptide ELISA
MDMeDIP
SHIn situ hybridization
IAEnzyme Immunoassay
SEsandwich ELISA
PLProximity Ligation Assay
ECELISA(Cap)
EDELISA(Det)
BIBioimaging
IOImmunoassay
LFLateral Flow Immunoassay
LALuminex Assay
CImmunohistochemistry-Frozen Sections
PImmunohistologyp-Paraffin Sections
ISIntracellular Staining for Flow Cytometry
MSElectrophoretic Mobility Shift Assay
RIRNA Binding Protein Immunoprecipitation (RIP)
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