Mouse Anti-ASTN1 Recombinant Antibody (H-9) (CBMAB-1154-CN)

Name: Mouse Anti-ASTN1 Recombinant Antibody (H-9)
SKU: CBMAB-1154-CN
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ASTN1 Recombinant Antibody (H-9) CBMAB-1154-CN in WB

Protein expression levels in 5 liver cancer cell lines as well as the MIHA normal liver cell line. ...View More

Chen, Q. F., Shi, F., Huang, T., Huang, C., Shen, L., Wu, P., & Li, W. (2020). ASTN1 is associated with immune infiltrates in hepatocellular carcinoma, and inhibits the migratory and invasive capacity of liver cancer via the Wnt/β‑catenin signaling pathway. Oncology Reports, 44(4), 1425-1440.

Mouse Anti-ASTN1 Recombinant Antibody (H-9) CBMAB-1154-CN in IHC

Immunohistochemistry staining of HCC tissues. T, tumor; NT, nontumor tissue. ...View More

Mouse Anti-ASTN1 Recombinant Antibody (H-9) CBMAB-1154-CN in IF

Immunofluorescence staining was performed to assess ASTN1 and β-catenin expression in xenografts. ASTN1 expression was increased and β-catenin expression was decreased in the ASTN1-overexpressing group. ...View More

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

H-9

Application

WB, ELISA, IF, IP

Immunogen

Amino acids 736-938 mapping within an internal region of Astrotactin of human origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Astrotactin 1

Introduction

Astrotactin is a neuronal adhesion molecule required for glial-guided migration of young postmitotic neuroblasts in cortical regions of developing brain, including cerebrum, hippocampus, cerebellum, and olfactory bulb. The protein is a neuronal adhesion molecule that is required for normal migration of young postmitotic neuroblasts along glial fibers, especially in the cerebellum. It is required for normal rate of migration of granule cells during brain development and for normal cerebellum development.

Entrez Gene ID

Human	460
Mouse	11899
Rat	304900

UniProt ID

Human	O14525
Mouse	Q61137
Rat	A0A1S3F323

Alternative Names

ASTN

Function

Neuronal adhesion molecule that is required for normal migration of young postmitotic neuroblasts along glial fibers, especially in the cerebellum. Required for normal rate of migration of granule cells during brain development and for normal cerebellum development.

Biological Process

Locomotory behavior Source: Ensembl
Neuron cell-cell adhesion Source: GO_Central
Neuron migration Source: GO_Central

Cellular Location

Endosome; Cell membrane; Perikaryon; Clathrin-coated vesicle. Detected close to the anterior pole and at the base of the leading process in migrating neurons. Is internalized from the membrane via clathrin-coated vesicles and endosomes, and recycled to the anterior pole of the migrating cell.

Topology

Extracellular: 22-153 aa
Helical: 154-174 aa
Cytoplasmic: 175-383 aa
Helical: 384-402 aa
Extracellular: 403-1302 aa

Mala, U. (2020). An integrative analysis of Cell Adhesion Molecules (CAMs) in Glioma: The Essential role of PTGFRN and ASTN1 in Glioma and Glioma Stem-like Cells (Doctoral dissertation).

Lara, P., Tellgren-Roth, Å., Behesti, H., Horn, Z., Schiller, N., Enquist, K., ... & Nilsson, I. (2019). Murine astrotactins 1 and 2 have a similar membrane topology and mature via endoproteolytic cleavage catalyzed by a signal peptidase. Journal of Biological Chemistry, 294(12), 4538-4545.

Lara, P., Tellgren-Roth, Å., Behesti, H., Horn, Z., Schiller, N., Enquist, K., ... & Nilsson, I. (2018). Astrotactins 1 and 2 have similar membrane topology and mature via endoproteolytic cleavage catalyzed by signal peptidase. bioRxiv, 493858.

Chang, H. (2017). Cleave but not leave: Astrotactin proteins in development and disease. IUBMB life, 69(8), 572-577.

Berkowicz, S. R., Giousoh, A., & Bird, P. I. (2017, December). Neurodevelopmental MACPFs: The vertebrate astrotactins and BRINPs. In Seminars in cell & developmental biology (Vol. 72, pp. 171-181). Academic Press.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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