Human ASTN1 ELISA Kit (V2LY-0626-LY3466)

Name: Human ASTN1 ELISA Kit
SKU: V2LY-0626-LY3466
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0066 ng/mL

Detection Range

0.015-3 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

144.9 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Astrotactin 1

Function

Neuronal adhesion molecule that is required for normal migration of young postmitotic neuroblasts along glial fibers, especially in the cerebellum. Required for normal rate of migration of granule cells during brain development and for normal cerebellum development.

Biological Process

Locomotory behavior Source: Ensembl
Neuron cell-cell adhesion Source: GO_Central
Neuron migration Source: GO_Central

Cellular Location

Endosome; Cell membrane; Perikaryon; Clathrin-coated vesicle. Detected close to the anterior pole and at the base of the leading process in migrating neurons. Is internalized from the membrane via clathrin-coated vesicles and endosomes, and recycled to the anterior pole of the migrating cell.

Topology

Extracellular: 22-153 aa
Helical: 154-174 aa
Cytoplasmic: 175-383 aa
Helical: 384-402 aa
Extracellular: 403-1302 aa

Chen, Q. F., Shi, F., Huang, T., Huang, C., Shen, L., Wu, P., & Li, W. (2020). ASTN1 is associated with immune infiltrates in hepatocellular carcinoma, and inhibits the migratory and invasive capacity of liver cancer via the Wnt/β‑catenin signaling pathway. Oncology reports, 44(4), 1425-1440.

Mala, U. (2020). An integrative analysis of Cell Adhesion Molecules (CAMs) in Glioma: The Essential role of PTGFRN and ASTN1 in Glioma and Glioma Stem-like Cells (Doctoral dissertation).

Lara, P., Tellgren-Roth, Å., Behesti, H., Horn, Z., Schiller, N., Enquist, K., ... & Nilsson, I. (2019). Murine astrotactins 1 and 2 have a similar membrane topology and mature via endoproteolytic cleavage catalyzed by a signal peptidase. Journal of Biological Chemistry, 294(12), 4538-4545.

Lara, P., Tellgren-Roth, Å., Behesti, H., Horn, Z., Schiller, N., Enquist, K., ... & Nilsson, I. (2018). Astrotactins 1 and 2 have similar membrane topology and mature via endoproteolytic cleavage catalyzed by signal peptidase. bioRxiv, 493858.

Chang, H. (2017). Cleave but not leave: Astrotactin proteins in development and disease. IUBMB life, 69(8), 572-577.

Berkowicz, S. R., Giousoh, A., & Bird, P. I. (2017, December). Neurodevelopmental MACPFs: The vertebrate astrotactins and BRINPs. In Seminars in cell & developmental biology (Vol. 72, pp. 171-181). Academic Press.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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