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ATG3

This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Full Name

Autophagy Related 3

Function

E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

Biological Process

Autophagosome assembly Source: UniProtKB
Autophagy of mitochondrion Source: GO_Central
Autophagy of nucleus Source: GO_Central
Cellular protein modification process Source: MGI
Macroautophagy Source: Reactome
Mitochondrial fragmentation involved in apoptotic process Source: UniProtKB
Negative regulation of phagocytosis Source: Ensembl
Protein targeting to membrane Source: UniProtKB
Protein ubiquitination Source: UniProtKB
Regulation of cilium assembly Source: UniProtKB

Cellular Location

Cytoplasm

PTM

Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy.
Cleaved by CASP8 upon death ligand binding such as tumor necrosis factor-alpha. CASP8 cleavage blocks survival-related autophagy and favors apoptosis.

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Anti-ATG3 antibodies

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Mouse Anti-ATG3 Recombinant Antibody (1F7) (CBMAB-A0572-LY)

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Target: ATG3

Host: Mouse

Antibody Isotype: IgG2b, κ

Specificity: Human

Clone: 1F7

Application*: WB, E

Mouse Anti-ATG3 Recombinant Antibody (4F6) (CBMAB-A3922-YC)

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Target: ATG3

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human, Mouse, Rat

Clone: 4F6

Application*: FC, IF, IH, WB

Mouse Anti-ATG3 Recombinant Antibody (3H2) (CBMAB-A3921-YC)

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Target: ATG3

Host: Mouse

Antibody Isotype: IgG2a

Specificity: Human, Dog, Rat, Monkey, Mouse

Clone: 3H2

Application*: WB, IH, IF, FC

Mouse Anti-ATG3 Recombinant Antibody (D9) (CBMAB-A3916-YC)

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Target: ATG3

Host: Mouse

Antibody Isotype: IgG1, κ

Specificity: Human, Mouse, Rat

Clone: D9

Application*: WB, IP, IF, E

Mouse Anti-ATG3 Recombinant Antibody (A6) (CBMAB-A3915-YC)

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Target: ATG3

Host: Mouse

Antibody Isotype: IgG2b, κ

Specificity: Human, Mouse, Rat

Clone: A6

Application*: WB, IP, E

Mouse Anti-ATG3 Recombinant Antibody (A3) (CBMAB-A3914-YC)

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Target: ATG3

Host: Mouse

Antibody Isotype: IgG2b, κ

Specificity: Human, Mouse, Rat

Clone: A3

Application*: WB, IP, IF, E

Mouse Anti-ATG3 Recombinant Antibody (10G1) (CBMAB-A3913-YC)

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Target: ATG3

Host: Mouse

Antibody Isotype: IgG2b, κ

Specificity: Human

Clone: 10G1

Application*: WB, E

Rabbit Anti-ATG3 Recombinant Antibody (SR1666) (CBMAB-1159-CN)

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Target: ATG3

Host: Rabbit

Antibody Isotype: IgG

Specificity: Human, Mouse, Rat

Clone: SR1666

Application*: IH, IF, WB

Mouse Anti-ATG3 Recombinant Antibody (CBT2245) (V2LY-0625-LY706)

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Target: ATG3

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human, Monkey

Clone: CBT2245

Application*: WB, IH, IC

Mouse Anti-ATG3 Recombinant Antibody (CBT2387) (V2LY-0625-LY705)

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Target: ATG3

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human

Clone: CBT2387

Application*: WB, IH, IC, F

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(P): Predicted

* Abbreviations

AActivation
AGAgonist
APApoptosis
BBlocking
BABioassay
BIBioimaging
CImmunohistochemistry-Frozen Sections
CIChromatin Immunoprecipitation
CTCytotoxicity
CSCostimulation
DDepletion
DBDot Blot

EELISA
ECELISA(Cap)
EDELISA(Det)
ESELISpot
EMElectron Microscopy
FFlow Cytometry
FNFunction Assay
GSGel Supershift
IInhibition
IAEnzyme Immunoassay
ICImmunocytochemistry
IDImmunodiffusion
IEImmunoelectrophoresis

IFImmunofluorescence
IGImmunochromatography
IHImmunohistochemistry
IMImmunomicroscopy
IOImmunoassay
IPImmunoprecipitation
ISIntracellular Staining for Flow Cytometry
LALuminex Assay
LFLateral Flow Immunoassay
MMicroarray
MCMass Cytometry/CyTOF
MDMeDIP

MSElectrophoretic Mobility Shift Assay
NNeutralization
PImmunohistologyp-Paraffin Sections
PAPeptide Array
PEPeptide ELISA
PLProximity Ligation Assay
RRadioimmunoassay
SStimulation
SESandwich ELISA
SHIn situ hybridization
TCTissue Culture
WBWestern Blot

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