Mouse Anti-ATG3 Recombinant Antibody (A6) (CBMAB-A3915-YC)

Name: Mouse Anti-ATG3 Recombinant Antibody (A6)
SKU: CBMAB-A3915-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ATG3 Recombinant Antibody (A6) (CBMAB-A3915-YC) in WB

Representative Western blot of mitophagy protein contents in TN and MCF-7 under normoxia (Nor, 21% O2) and 24 h hypoxia (Hyp, 0.1% O2). Normalization was performed by comparing to the α-tubulin protein levels and using the result from the normoxia sample set as the relative control value. Data shown represents the mean ± S.D. of at least three different preparations. * P <0.05 vs. Nor; ** P <0.05 vs. MCF-7. ...View More

Pacheco-Velázquez, S. C., Robledo-Cadena, D. X., Hernández-Reséndiz, I., Gallardo-Pérez, J. C., Moreno-Sánchez, R., & Rodríguez-Enríquez, S. (2018). Energy metabolism drugs block triple negative breast metastatic cancer cell phenotype. Molecular pharmaceutics, 15(6), 2151-2164.

Mouse Anti-ATG3 Recombinant Antibody (A6) (CBMAB-A3915-YC) in WB

Western blot analysis to evaluate the levels of autophagy-related protein LC3B. ...View More

Wang, H., Zhang, Y., Wu, Q., Wang, Y. B., & Wang, W. (2018). miR-16 mimics inhibit TGF-β1-induced epithelial-to-mesenchymal transition via activation of autophagy in non-small cell lung carcinoma cells. Oncology reports, 39(1), 247-254.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

Application

WB, IP, ELISA

Immunogen

Recombinant Atg3 of human origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Autophagy Related 3

Introduction

ATG3 is a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role i

Entrez Gene ID

Human	64422
Mouse	67841

UniProt ID

Human	Q9NT62
Mouse	Q9CPX6

Alternative Names

Autophagy Related 3; Autophagy-Related Protein 3; APG3-LIKE; APG3L; HApg3; APG3; ATG3 Autophagy Related 3 Homolog (S. Cerevisiae); Ubiquitin-Like-Conjugating Enzyme ATG3;

Function

E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

Biological Process

Autophagosome assembly Source: UniProtKB
Autophagy of mitochondrion Source: GO_Central
Autophagy of nucleus Source: GO_Central
Cellular protein modification process Source: MGI
Macroautophagy Source: Reactome
Mitochondrial fragmentation involved in apoptotic process Source: UniProtKB
Negative regulation of phagocytosis Source: Ensembl
Protein targeting to membrane Source: UniProtKB
Protein ubiquitination Source: UniProtKB
Regulation of cilium assembly Source: UniProtKB

Cellular Location

Cytoplasm

PTM

Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy.
Cleaved by CASP8 upon death ligand binding such as tumor necrosis factor-alpha. CASP8 cleavage blocks survival-related autophagy and favors apoptosis.

Zhou, K., Xu, Y., Wang, Q., & Dong, L. (2021). Overexpression of miR-431 attenuates hypoxia/reoxygenation-induced myocardial damage via autophagy-related 3. Acta Biochimica et Biophysica Sinica, 53(2), 140-148.

Peng, Z., Ji, D., Qiao, L., Chen, Y., & Huang, H. (2021). Autophagy Inhibition by ATG3 Knockdown Remits Oxygen–Glucose Deprivation/Reoxygenation-Induced Injury and Inflammation in Brain Microvascular Endothelial Cells. Neurochemical Research, 1-13.

Ismayil, A., Yang, M., Haxim, Y., Wang, Y., Li, J., Han, L., ... & Liu, Y. (2020). Cotton leaf curl Multan virus βC1 protein induces autophagy by disrupting the interaction of autophagy-related protein 3 with glyceraldehyde-3-phosphate dehydrogenases. The Plant Cell, 32(4), 1124-1135.

Yang, L., Peng, X., Jin, H., & Liu, J. (2019). Long non-coding RNA PVT1 promotes autophagy as ceRNA to target ATG3 by sponging microRNA-365 in hepatocellular carcinoma. Gene, 697, 94-102.

Kang, Y., Jia, Y., Wang, Q., Zhao, Q., Song, M., Ni, R., & Wang, J. (2019). Long noncoding RNA KCNQ1OT1 promotes the progression of non-small cell lung cancer via regulating miR-204-5p/ATG3 axis. OncoTargets and therapy, 12, 10787.

Zhang, Y., Li, F., Liu, L., Jiang, H., Jiang, X., Ge, X., ... & Wang, Y. (2018). Salinomycin-induced autophagy blocks apoptosis via the ATG3/AKT/mTOR signaling axis in PC-3 cells. Life sciences, 207, 451-460.

Hua, L., Zhu, G., & Wei, J. (2018). MicroRNA‐1 overexpression increases chemosensitivity of non‐small cell lung cancer cells by inhibiting autophagy related 3‐mediated autophagy. Cell biology international, 42(9), 1240-1249.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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