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Rabbit Anti-ATG3 Recombinant Antibody (SR1666) (CBMAB-1159-CN)

This product is a rabbit antibody that recognizes ATG3 of human. The antibody EPR4801 can be used for immunoassay techniques such as: FC, IF, IHC-P, WB.
See all ATG3 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
SR1666
Antibody Isotype
IgG
Application
IHC, IF, WB

Basic Information

Immunogen
A synthesized peptide derived from human ATG3/APG3.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:500-1:2,000
IF(ICC)1:50-1:200
IHC1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Autophagy Related 3
Introduction
This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. This protein catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy.
Entrez Gene ID
Human64422
Mouse67841
Rat171415
UniProt ID
HumanQ9NT62
MouseQ9CPX6
RatQ6AZ50
Alternative Names
APG3; APG3L; PC3-96; APG3-LIKE
Function
E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.
Biological Process
Autophagosome assembly Source: UniProtKB
Autophagy of mitochondrion Source: GO_Central
Autophagy of nucleus Source: GO_Central
Cellular protein modification process Source: MGI
Macroautophagy Source: Reactome
Mitochondrial fragmentation involved in apoptotic process Source: UniProtKB
Negative regulation of phagocytosis Source: Ensembl
Protein targeting to membrane Source: UniProtKB
Protein ubiquitination Source: UniProtKB
Regulation of cilium assembly Source: UniProtKB
Cellular Location
Cytoplasm
PTM
Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy.
Cleaved by CASP8 upon death ligand binding such as tumor necrosis factor-alpha. CASP8 cleavage blocks survival-related autophagy and favors apoptosis.

Zhou, K., Xu, Y., Wang, Q., & Dong, L. (2021). Overexpression of miR-431 attenuates hypoxia/reoxygenation-induced myocardial damage via autophagy-related 3. Acta Biochimica et Biophysica Sinica, 53(2), 140-148.

Peng, Z., Ji, D., Qiao, L., Chen, Y., & Huang, H. (2021). Autophagy Inhibition by ATG3 Knockdown Remits Oxygen–Glucose Deprivation/Reoxygenation-Induced Injury and Inflammation in Brain Microvascular Endothelial Cells. Neurochemical Research, 1-13.

Ismayil, A., Yang, M., Haxim, Y., Wang, Y., Li, J., Han, L., ... & Liu, Y. (2020). Cotton leaf curl Multan virus βC1 protein induces autophagy by disrupting the interaction of autophagy-related protein 3 with glyceraldehyde-3-phosphate dehydrogenases. The Plant Cell, 32(4), 1124-1135.

Yang, L., Peng, X., Jin, H., & Liu, J. (2019). Long non-coding RNA PVT1 promotes autophagy as ceRNA to target ATG3 by sponging microRNA-365 in hepatocellular carcinoma. Gene, 697, 94-102.

Kang, Y., Jia, Y., Wang, Q., Zhao, Q., Song, M., Ni, R., & Wang, J. (2019). Long noncoding RNA KCNQ1OT1 promotes the progression of non-small cell lung cancer via regulating miR-204-5p/ATG3 axis. OncoTargets and therapy, 12, 10787.

Zhang, Y., Li, F., Liu, L., Jiang, H., Jiang, X., Ge, X., ... & Wang, Y. (2018). Salinomycin-induced autophagy blocks apoptosis via the ATG3/AKT/mTOR signaling axis in PC-3 cells. Life sciences, 207, 451-460.

Hua, L., Zhu, G., & Wei, J. (2018). MicroRNA‐1 overexpression increases chemosensitivity of non‐small cell lung cancer cells by inhibiting autophagy related 3‐mediated autophagy. Cell biology international, 42(9), 1240-1249.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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