CD209 Antibodies

Structure of CD209

The molecular weight of CD209 protein is approximately 45 kDa, and there are differences among different species. The extracellular segment of this protein contains a C-type lectin domain, which mediates pathogen recognition.

The CD209 protein is composed of 404 amino acids and is a transmembrane receptor protein. Its structure consists of three main regions: the N-terminal cytoplasmic region, the transmembrane region, and the C-terminal extracellular region. The extracellular region contains a characteristic C-type lectin-like domain (CRD), which maintains its globular conformation through two disulfide bonds. The interior of this domain contains a conserved EPN motif (Glu-Pro-Asn), forming a ligand-binding site that specifically recognizes the mannose and fucose structures on the surface of pathogens. The CRD structure coordinates and binds two calcium ions, one to maintain structural stability and the other to directly participate in sugar recognition. Quaternization is the functional form of CD209 for recognizing multivalent ligands. Each subunit wraps around each other through the coiled-coil structure in the neck region to form a stalk-like structure, allowing the CRD to extend outward to capture the pathogen.

Fig. 1 CD209 family proteins.¹

Key structural properties of CD209:

• Transmembrane receptor structure
• Extracellular region forms a tetramer
• Calcium ion-dependent recognition of pathogen surface carbohydrate structures
• Intracellular tail region contains an endocytosis motif, mediating antigen uptake and presentation

Functions of CD209

The main function of CD209 is to recognize the sugar structures on the surface of pathogens and trigger the immune response. It is also involved in various physiological processes such as immune regulation and cell migration.

The ligand-binding characteristics of CD209 are calcium ion-dependent and exhibit a specific recognition pattern with the mannose-type structure, reflecting its crucial role in pathogen capture and its functional positioning for initiating rapid immune responses.

Applications of CD209 and CD209 Antibody in Literature

1. Rahimi, Nader. "C-type lectin CD209L/L-SIGN and CD209/DC-SIGN: cell adhesion molecules turned to pathogen recognition receptors." Biology 10.1 (2020): 1. https://doi.org/10.3390/biology10010001

During the COVID-19 pandemic, the CD209 family proteins have attracted much attention as viral receptors. They mediate invasion by recognizing the viral spike protein and are widely expressed in target organs with genetic polymorphisms. Studying these interactions is of great significance for understanding the pathogenic mechanism and developing vaccines.

2. Lin, Anqi, et al. "CD209 signaling pathway as a biomarker for cisplatin chemotherapy response in small cell lung cancer." Genes & Diseases 11.3 (2023): 101038. https://doi.org/10.1016/j.gendis.2023.06.011

The research has found that small cell lung cancer is prone to developing resistance to cisplatin. The high expression of CD209 in lung cancer indicates a better prognosis, and its signaling pathway may affect the chemotherapy outcome by regulating immunity. Studying the relationship between CD209 and the efficacy and prognosis of cisplatin treatment is of great significance for overcoming drug resistance.

3. Marzaioli, Viviana, et al. "CD209/CD14+ dendritic cells characterization in rheumatoid and psoriatic arthritis patients: activation, synovial infiltration, and therapeutic targeting." Frontiers in immunology 12 (2022): 722349. https://doi.org/10.3389/fimmu.2021.722349

The study found that there was an increase in CD209/CD14+ dendritic cells in the bodies of arthritis patients, which exhibited a unique inflammatory phenotype. Joint fluid can induce their formation, and the JAK/STAT pathway regulates their functions. Inhibiting this pathway may alleviate inflammation and provide a new strategy for treatment.

4. Chang, Kai, et al. "Association between CD209-336A/G and-871A/G polymorphisms and susceptibility of tuberculosis: a meta-analysis." PLoS One 7.7 (2012): e41519. https://doi.org/10.1371/journal.pone.0041519

The meta-analysis showed that the polymorphisms at positions -336 and -871 of the CD209 promoter were not associated with the overall risk of tuberculosis. However, the GG genotype at the -336 locus might increase the tuberculosis susceptibility in the Asian population, providing a basis for the genetic risk of specific populations.

5. Shadrina, Alexandra S., et al. "Mendelian randomization analysis of plasma levels of CD209 and MICB proteins and the risk of varicose veins of lower extremities." Plos one 17.5 (2022): e0268725. https://doi.org/10.1371/journal.pone.0268725

The Mendelian randomization study found that the elevated level of CD209 protein predicted by genes was significantly associated with an increased risk of lower extremity varicose veins (OR = 1.08). This result supports the possibility that CD209 may be involved in the pathogenesis of varicose veins, providing genetic evidence for subsequent mechanism studies.

Creative Biolabs: CD209 Antibodies for Research

Creative Biolabs specializes in the production of high-quality CD209 antibodies for research and industrial applications. Our portfolio includes monoclonal and polyclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom CD209 Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our CD209 antibodies, custom preparations, or technical support, contact us at email.