LIMA1

This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated.

LIM Domain And Actin Binding 1

Actin-binding protein involved in actin cytoskeleton regulation and dynamics. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments (PubMed:12566430).
Plays a role in cholesterol homeostasis. Influences plasma cholesterol levels through regulation of intestinal cholesterol absorption. May act as a scaffold protein by regulating NPC1L1 transportation, an essential protein for cholesterol absorption, to the plasma membrane by recruiting MYO5B to NPC1L1, and thus facilitates cholesterol uptake (By similarity).

Actin filament bundle assemblyManual Assertion Based On ExperimentIDA:UniProtKB
Cell migrationISS:UniProtKB
Cholesterol homeostasisISS:UniProtKB
Cholesterol metabolic processIEA:UniProtKB-KW
Intestinal cholesterol absorptionISS:UniProtKB
Negative regulation of actin filament depolymerizationManual Assertion Based On ExperimentIDA:UniProtKB
Ruffle organizationManual Assertion Based On ExperimentIDA:UniProtKB

Cytoplasm
Cell junction, focal adhesion
Cytoplasm, cytoskeleton
Cytoplasm, cytoskeleton, stress fiber
Cell membrane
Expressed in the brush border membrane of the small intestine and colocalizes with NPC1L1 and MYO5B (PubMed:29880681).
Colocalizes with PXN at focal adhesions in mesangial cells (PubMed:24694988).
Colocalizes with actin stress fibers in quiescent cells. PDGF stimulation induced disassembly of stress fibers and formation of peripheral and dorsal ruffles, where LIMA1 is relocalized (By similarity).

Phosphorylation of the C-terminal region by MAPK1/MAPK3 reduces its association with F-actin and contributes to actin filament reorganization and enhances cell motility.