Mouse Anti-LIMA1 Recombinant Antibody (CBYCL-331) (CBMAB-L0232-YC)

Name: Mouse Anti-LIMA1 Recombinant Antibody (CBYCL-331)
SKU: CBMAB-L0232-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYCL-331

Application

IHC-P, ELISA, WB

Immunogen

Recombiant full length LIMA1 protein

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH7.4

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 1-598

More Infomation

Target

Full Name

LIM Domain And Actin Binding 1

Introduction

LIMA1 is a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Among its related pathways are Cytoskeletal Signaling and Stabilization and expansion of the E-cadherin adherens junction.

Entrez Gene ID

51474

UniProt ID

Q9UHB6

Alternative Names

EPLIN; SREBP3

Function

Actin-binding protein involved in actin cytoskeleton regulation and dynamics. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments (PubMed:12566430).
Plays a role in cholesterol homeostasis. Influences plasma cholesterol levels through regulation of intestinal cholesterol absorption. May act as a scaffold protein by regulating NPC1L1 transportation, an essential protein for cholesterol absorption, to the plasma membrane by recruiting MYO5B to NPC1L1, and thus facilitates cholesterol uptake (By similarity).

Biological Process

Actin filament bundle assemblyManual Assertion Based On ExperimentIDA:UniProtKB
Cell migrationISS:UniProtKB
Cholesterol homeostasisISS:UniProtKB
Cholesterol metabolic processIEA:UniProtKB-KW
Intestinal cholesterol absorptionISS:UniProtKB
Negative regulation of actin filament depolymerizationManual Assertion Based On ExperimentIDA:UniProtKB
Ruffle organizationManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Cytoplasm
Cell junction, focal adhesion
Cytoplasm, cytoskeleton
Cytoplasm, cytoskeleton, stress fiber
Cell membrane
Expressed in the brush border membrane of the small intestine and colocalizes with NPC1L1 and MYO5B (PubMed:29880681).
Colocalizes with PXN at focal adhesions in mesangial cells (PubMed:24694988).
Colocalizes with actin stress fibers in quiescent cells. PDGF stimulation induced disassembly of stress fibers and formation of peripheral and dorsal ruffles, where LIMA1 is relocalized (By similarity).

PTM

Phosphorylation of the C-terminal region by MAPK1/MAPK3 reduces its association with F-actin and contributes to actin filament reorganization and enhances cell motility.

Wang, X., Zhang, C., Song, H., Yuan, J., Zhang, X., Yuan, Y., ... & He, J. (2023). Characterization of LIMA1 and its emerging roles and potential therapeutic prospects in cancers. Frontiers in Oncology, 13, 1115943.

Jiang, W., Yang, X., Shi, K., Zhang, Y., Shi, X., Wang, J., ... & Li, X. (2023). MAD2 activates IGF1R/PI3K/AKT pathway and promotes cholangiocarcinoma progression by interfering USP44/LIMA1 complex. Oncogene, 42(45), 3344-3357.

Qi, Y., Wang, H., Zhang, Q., Liu, Z., Wang, T., Wu, Z., & Wu, W. (2022). CAF-released exosomal miR-20a-5p facilitates HCC progression via the LIMA1-mediated β-catenin pathway. Cells, 11(23), 3857.

Duethorn, B., Groll, F., Rieger, B., Drexler, H. C., Brinkmann, H., Kremer, L., ... & Bedzhov, I. (2022). Lima1 mediates the pluripotency control of membrane dynamics and cellular metabolism. Nature Communications, 13(1), 610.

Ma, W., Liao, Y., Gao, Z., Zhu, W., Liu, J., & She, W. (2022). Overexpression of LIMA1 indicates poor prognosis and promotes epithelial-mesenchymal transition in head and neck squamous cell carcinoma. Clinical Medicine Insights: Oncology, 16, 11795549221109493.

Huang, H., Du, Y., Zhao, D., & Chen, K. (2022). The Relationship between the Prognostic Marker LIMA1 in Head and Neck Squamous Cell Carcinoma and Immune Infiltration. Journal of Oncology, 2022.

Hirano, S., Yamamoto, T., & Michiue, T. (2018). FRET-based tension measurement across actin-associated mechanotransductive structures using Lima1. International Journal of Developmental Biology, 62(9-10), 631-636.

Lim, G. B. (2018). LIMA1 variant influences cholesterol absorption. Nature Reviews Cardiology, 15(9), 502-502.

Zhang, Y. Y., Fu, Z. Y., Wei, J., Qi, W., Baituola, G., Luo, J., ... & Song, B. L. (2018). A LIMA1 variant promotes low plasma LDL cholesterol and decreases intestinal cholesterol absorption. Science, 360(6393), 1087-1092.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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