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Mouse Anti-LIMA1 (AA 1-275) Recombinant Antibody (CBFYE-1479) (CBMAB-E2112-FY)

This product is mouse antibody that recognizes LIMA1. The antibody CBFYE-1479 can be used for immunoassay techniques such as: ELISA, WB, IF, IHC.
See all LIMA1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYE-1479
Antibody Isotype
IgG1
Application
ELISA, WB, IF, IHC

Basic Information

Immunogen
Human recombinant protein fragment corresponding to amino acids 1-275 of human LIMA1 produced in E.coli
Specificity
Human
Antibody Isotype
IgG1
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
>95% by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 1-275

Target

Full Name
LIM Domain And Actin Binding 1
Introduction
This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines.
Entrez Gene ID
51474
UniProt ID
Q9UHB6
Alternative Names
EPLIN; SREBP3
Function
Actin-binding protein involved in actin cytoskeleton regulation and dynamics. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments (PubMed:12566430).
Plays a role in cholesterol homeostasis. Influences plasma cholesterol levels through regulation of intestinal cholesterol absorption. May act as a scaffold protein by regulating NPC1L1 transportation, an essential protein for cholesterol absorption, to the plasma membrane by recruiting MYO5B to NPC1L1, and thus facilitates cholesterol uptake (By similarity).
Biological Process
Actin filament bundle assemblyManual Assertion Based On ExperimentIDA:UniProtKB
Cell migrationISS:UniProtKB
Cholesterol homeostasisISS:UniProtKB
Cholesterol metabolic processIEA:UniProtKB-KW
Intestinal cholesterol absorptionISS:UniProtKB
Negative regulation of actin filament depolymerizationManual Assertion Based On ExperimentIDA:UniProtKB
Ruffle organizationManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Cytoplasm
Cell junction, focal adhesion
Cytoplasm, cytoskeleton
Cytoplasm, cytoskeleton, stress fiber
Cell membrane
Expressed in the brush border membrane of the small intestine and colocalizes with NPC1L1 and MYO5B (PubMed:29880681).
Colocalizes with PXN at focal adhesions in mesangial cells (PubMed:24694988).
Colocalizes with actin stress fibers in quiescent cells. PDGF stimulation induced disassembly of stress fibers and formation of peripheral and dorsal ruffles, where LIMA1 is relocalized (By similarity).
PTM
Phosphorylation of the C-terminal region by MAPK1/MAPK3 reduces its association with F-actin and contributes to actin filament reorganization and enhances cell motility.

Wang, X., Zhang, C., Song, H., Yuan, J., Zhang, X., Yuan, Y., ... & He, J. (2023). Characterization of LIMA1 and its emerging roles and potential therapeutic prospects in cancers. Frontiers in Oncology, 13, 1115943.

Jiang, W., Yang, X., Shi, K., Zhang, Y., Shi, X., Wang, J., ... & Li, X. (2023). MAD2 activates IGF1R/PI3K/AKT pathway and promotes cholangiocarcinoma progression by interfering USP44/LIMA1 complex. Oncogene, 42(45), 3344-3357.

Qi, Y., Wang, H., Zhang, Q., Liu, Z., Wang, T., Wu, Z., & Wu, W. (2022). CAF-released exosomal miR-20a-5p facilitates HCC progression via the LIMA1-mediated β-catenin pathway. Cells, 11(23), 3857.

Duethorn, B., Groll, F., Rieger, B., Drexler, H. C., Brinkmann, H., Kremer, L., ... & Bedzhov, I. (2022). Lima1 mediates the pluripotency control of membrane dynamics and cellular metabolism. Nature Communications, 13(1), 610.

Ma, W., Liao, Y., Gao, Z., Zhu, W., Liu, J., & She, W. (2022). Overexpression of LIMA1 indicates poor prognosis and promotes epithelial-mesenchymal transition in head and neck squamous cell carcinoma. Clinical Medicine Insights: Oncology, 16, 11795549221109493.

Huang, H., Du, Y., Zhao, D., & Chen, K. (2022). The Relationship between the Prognostic Marker LIMA1 in Head and Neck Squamous Cell Carcinoma and Immune Infiltration. Journal of Oncology, 2022.

Hirano, S., Yamamoto, T., & Michiue, T. (2018). FRET-based tension measurement across actin-associated mechanotransductive structures using Lima1. International Journal of Developmental Biology, 62(9-10), 631-636.

Lim, G. B. (2018). LIMA1 variant influences cholesterol absorption. Nature Reviews Cardiology, 15(9), 502-502.

Zhang, Y. Y., Fu, Z. Y., Wei, J., Qi, W., Baituola, G., Luo, J., ... & Song, B. L. (2018). A LIMA1 variant promotes low plasma LDL cholesterol and decreases intestinal cholesterol absorption. Science, 360(6393), 1087-1092.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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