NEK11

This gene encodes a member of the never in mitosis gene A family of kinases. The encoded protein localizes to the nucleoli, and may function with NEK2A in the S-phase checkpoint. The encoded protein appears to play roles in DNA replication and response to genotoxic stress. Alternatively spliced transcript variants have been described.[provided by RefSeq, Mar 2009]

Full Name

NIMA Related Kinase 11

Function

Protein kinase which plays an important role in the G2/M checkpoint response to DNA damage. Controls degradation of CDC25A by directly phosphorylating it on residues whose phosphorylation is required for BTRC-mediated polyubiquitination and degradation.

Biological Process

Chromosome segregation Source: GO_Central
Histone phosphorylation Source: CACAO
Intracellular signal transduction Source: UniProtKB
Mitotic intra-S DNA damage checkpoint signaling Source: UniProtKB
Protein phosphorylation Source: UniProtKB
Regulation of mitotic cell cycle phase transition Source: CACAO

Cellular Location

Nucleus
nucleolus
Note: Nuclear during interphase but moves to the polar microtubules during prometaphase and metaphase (PubMed:12154088). Accumulates in the nucleolus in G1/S-arrested cells (PubMed:15161910).

PTM

Phosphorylated by NEK2. Phosphorylation at Ser-273 is important for its activation.

Anti-NEK11 antibodies

Mouse Anti-NEK11 Recombinant Antibody (4E1-1F4) (CBMAB-A5869-LY)

Datasheet

SDS

Target: NEK11

Host: Mouse

Antibody Isotype: IgG1, κ

Specificity: Human

Clone: 4E1-1F4

Application*: WB, E

Mouse Anti-NEK11 Recombinant Antibody (5D9) (CBMAB-N1920-WJ)

Datasheet

SDS

Target: NEK11

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human

Clone: 5D9

Application*: WB, IH, IF

Mouse Anti-NEK11 Recombinant Antibody (4H5) (CBMAB-N1919-WJ)

Datasheet

SDS

Target: NEK11

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human

Clone: 4H5

Application*: WB, IF

Mouse Anti-NEK11 Recombinant Antibody (CBWJN-0416) (CBMAB-N1918-WJ)

Datasheet

SDS

Target: NEK11

Host: Mouse

Antibody Isotype: IgG1, κ

Specificity: Human

Clone: CBWJN-0416

Application*: E, IF, IH, WB

Mouse Anti-NEK11 Recombinant Antibody (5E9) (CBMAB-N1917-WJ)

Datasheet

SDS

Target: NEK11

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human

Clone: 5E9

Application*: F, WB

NEK11 Matched Antibody Pair (790) (APMAB-790LY)

Datasheet

SDS

Target: NEK11

Specificity: Human

NEK11 Matched Antibody Pair (789) (APMAB-789LY)

Datasheet

SDS

Target: NEK11

Specificity: Human

For Research Use Only. Not For Clinical Use.

(P): Predicted

* Abbreviations

IFImmunofluorescence

IHImmunohistochemistry

IPImmunoprecipitation

WBWestern Blot

EELISA

MMicroarray

CIChromatin Immunoprecipitation

FFlow Cytometry

FNFunction Assay

IDImmunodiffusion

RRadioimmunoassay

TCTissue Culture

GSGel Supershift

NNeutralization

BBlocking

AActivation

IInhibition

DDepletion

ESELISpot

DBDot Blot

MCMass Cytometry/CyTOF

CTCytotoxicity

SStimulation

AGAgonist

APApoptosis

IMImmunomicroscopy

BABioassay

CSCostimulation

EMElectron Microscopy

IEImmunoelectrophoresis

PAPeptide Array

ICImmunocytochemistry

PEPeptide ELISA

MDMeDIP

SHIn situ hybridization

IAEnzyme Immunoassay

SEsandwich ELISA

PLProximity Ligation Assay

ECELISA(Cap)

EDELISA(Det)

BIBioimaging

IOImmunoassay

LFLateral Flow Immunoassay

LALuminex Assay

CImmunohistochemistry-Frozen Sections

PImmunohistologyp-Paraffin Sections

ISIntracellular Staining for Flow Cytometry

MSElectrophoretic Mobility Shift Assay

RIRNA Binding Protein Immunoprecipitation (RIP)