Mouse Anti-NEK11 Recombinant Antibody (4E1-1F4) (CBMAB-A5869-LY)

Name: Mouse Anti-NEK11 Recombinant Antibody (4E1-1F4)
SKU: CBMAB-A5869-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4E1-1F4

Application

WB, ELISA

Immunogen

NEK11 (AAH28587, 1 a.a. ~ 482 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

NIMA Related Kinase 11

Introduction

This gene encodes a member of the never in mitosis gene A family of kinases. The encoded protein localizes to the nucleoli, and may function with NEK2A in the S-phase checkpoint. The encoded protein appears to play roles in DNA replication and response to genotoxic stress. Alternatively spliced transcript variants have been described

Entrez Gene ID

79858

UniProt ID

Q8NG66

Alternative Names

FLJ23495

Function

Protein kinase which plays an important role in the G2/M checkpoint response to DNA damage. Controls degradation of CDC25A by directly phosphorylating it on residues whose phosphorylation is required for BTRC-mediated polyubiquitination and degradation.

Biological Process

Chromosome segregation Source: GO_Central
Histone phosphorylation Source: CACAO
Intracellular signal transduction Source: UniProtKB
Mitotic intra-S DNA damage checkpoint signaling Source: UniProtKB
Protein phosphorylation Source: UniProtKB
Regulation of mitotic cell cycle phase transition Source: CACAO

Cellular Location

Nucleus
nucleolus
Note: Nuclear during interphase but moves to the polar microtubules during prometaphase and metaphase (PubMed:12154088). Accumulates in the nucleolus in G1/S-arrested cells (PubMed:15161910).

PTM

Phosphorylated by NEK2. Phosphorylation at Ser-273 is important for its activation.

Christodoulou, E., van Doorn, R., Visser, M., Teunisse, A., Versluis, M., van der Velden, P., ... & Gruis, N. (2020). NEK11 as a candidate high-penetrance melanoma susceptibility gene. Journal of Medical Genetics, 57(3), 203-210.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

NEK11 Matched Antibody Pair (789) (CAT#: APMAB-789LY)

NEK11 Matched Antibody Pair (790) (CAT#: APMAB-790LY)

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NEK11 Matched Antibody Pair (789) (CAT#: APMAB-789LY)

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NEK11 Matched Antibody Pair (790) (CAT#: APMAB-790LY)

Mouse Anti-NEK11 Recombinant Antibody (5D9) (CAT#: CBMAB-N1920-WJ)

Mouse Anti-NEK11 Recombinant Antibody (5E9) (CAT#: CBMAB-N1917-WJ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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