NGFR Antibodies

Structure of NGFR

NGFR is a type I transmembrane glycoprotein with a molecular weight of approximately 45 kDa. Its size varies slightly among different species due to differences in glycosylation. The primary structure of the NGFR protein consists of an extracellular domain, a transmembrane region, and an intracellular domain, each containing approximately 400 amino acids. The extracellular domain is rich in four conserved cysteine repeat sequences, forming a typical ligand-binding domain that specifically recognizes and binds neurotrophic factors. The secondary structure of this protein is mainly composed of β-sheets, collectively forming a stable ligand-binding interface. Although the intracellular domain lacks kinase activity, it recruits downstream signal adaptor proteins through its death domain, thereby initiating cell survival or apoptosis signaling pathways.

Fig. 1 Structure of NGFR/p75NTR isoforms.¹

Key structural properties of NGFR:

• Type I transmembrane protein structure
• Extracellular ligand binding domains rich in cysteine repeat sequences
• Intracellular death domains lacking kinase activity

Functions of NGFR

The main function of the NGFR gene is to mediate the signal transduction of neurotrophic factors, which is crucial for the survival, differentiation and synaptic plasticity of neurons. At the same time, it is also involved in various pathological physiological processes.

Unlike typical receptors with a single high-affinity binding property, NGFR (especially p75NTR) can bind to multiple neurotrophic factors with lower affinity and often forms complexes with Trk receptors. Its signal output is highly context-dependent, capable of promoting survival as well as inducing apoptosis.

Applications of NGFR and NGFR Antibody in Literature

1. Bruno, Francesco, et al. "The nerve growth factor receptor (NGFR/p75NTR): A major player in Alzheimer's disease." International journal of molecular sciences 24.4 (2023): 3200. https://doi.org/10.3390/ijms24043200

The article indicates that NGFR/p75NTR, as a common receptor for neurotrophic factors and β-amyloid protein (Aβ), plays a crucial role in the pathological process, genetic mechanism, and diagnostic and therapeutic strategies of Alzheimer's disease. This article systematically reviews its multi-level functions in the disease.

2. Chen, Hao, et al. "NGFR increases the chemosensitivity of colorectal cancer cells by enhancing the apoptotic and autophagic effects of 5-fluorouracil via the activation of S100A9." Frontiers in Oncology 11 (2021): 652081. https://doi.org/10.3389/fonc.2021.652081

The article indicates that the high expression of nerve growth factor receptor (NGFR) in colorectal cancer can enhance the sensitivity of cancer cells to 5-FU. The mechanism may be related to the induction of apoptosis, autophagy, and regulation of pathways such as S100A9, suggesting that NGFR may serve as a potential biomarker for predicting the efficacy of chemotherapy.

3. Boshuizen, Julia, et al. "Reversal of pre-existing NGFR-driven tumor and immune therapy resistance." Nature communications 11.1 (2020): 3946. https://doi.org/10.1038/s41467-020-17739-8

This study has confirmed the existence of a pre-existing and stable subgroup with high expression of NGFR in melanoma. This subgroup is resistant to both T cells and targeted therapies, and predicts resistance to PD-1 therapy. Inhibiting NGFR can restore the tumor's sensitivity to T cells, providing a potential target for overcoming immune therapy resistance.

4. Xie, Ning, et al. "Efficient muscle regeneration by human PSC-derived CD82+ ERBB3+ NGFR+ skeletal myogenic progenitors." Cells 12.3 (2023): 362. https://doi.org/10.3390/cells12030362

The article indicates that by using the CD82, ERBB3 and NGFR markers, highly regenerative skeletal muscle progenitor cells can be efficiently isolated from teratoma derived from human stem cells. These cells can be expanded in vitro and, after transplantation, can efficiently regenerate muscle fibers and replenish the stem cell bank.

5. Lehmann, Julia, et al. "Escape from NK cell tumor surveillance by NGFR-induced lipid remodeling in melanoma." Science advances 9.2 (2023): eadc8825. https://doi.org/10.1126/sciadv.adc8825

The article indicates that the expression of NGFR enables melanoma cells to evade immune surveillance by natural killer cells. The mechanism involves down-regulating activating ligands and up-regulating the SCD enzyme. Inhibiting SCD can reverse the immune escape mediated by NGFR, providing a new target for the prevention and treatment of metastasis.

Creative Biolabs: NGFR Antibodies for Research

Creative Biolabs specializes in the production of high-quality NGFR antibodies for research and industrial applications. Our portfolio includes monoclonal and polyclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom NGFR Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our NGFR antibodies, custom preparations, or technical support, contact us at email.