RANGAP1

This gene encodes a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. The encoded protein interacts with Ras-related nuclear protein 1 (RAN) and regulates guanosine triphosphate (GTP)-binding and exchange. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Full Name

Ran GTPase Activating Protein 1

Function

GTPase activator for RAN (PubMed:8146159, PubMed:8896452, PubMed:16428860).
Converts cytoplasmic GTP-bound RAN to GDP-bound RAN, which is essential for RAN-mediated nuclear import and export (PubMed:8896452, PubMed:27160050).
Mediates dissociation of cargo from nuclear export complexes containing XPO1, RAN and RANBP2 after nuclear export (PubMed:27160050).

Biological Process

Biological Process activation of GTPase activityManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process cellular response to vasopressinIEA:Ensembl
Biological Process negative regulation of protein export from nucleusManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process nuclear export1 PublicationIC:ComplexPortal
Biological Process nucleocytoplasmic transportManual Assertion Based On ExperimentIBA:GO_Central
Biological Process protein sumoylationManual Assertion Based On ExperimentIDA:ComplexPortal
Biological Process response to axon injuryIEA:Ensembl
Biological Process signal transductionManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Cytoplasm
Nucleus, nucleoplasm
Nucleus envelope
Chromosome, centromere, kinetochore
Cytoplasm, cytoskeleton, spindle
Cytoplasmic during interphase. Detected at the nuclear envelope during interphase (PubMed:11854305, PubMed:15037602).
Targeted to the nuclear pores after sumoylation (PubMed:11854305).
During mitosis, associates with mitotic spindles, but is essentially not detected at the spindle poles (PubMed:11854305, PubMed:15037602).
Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase (PubMed:11854305).
Mitotic location also requires sumoylation (PubMed:11854305).

PTM

Phosphorylation occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBE2I, on nuclear pore complex (NPC) diassembly and during mitosis.
Sumoylated (PubMed:11854305, PubMed:15037602, PubMed:26304119, PubMed:27160050).
Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis (PubMed:11854305).
Also required for interaction with RANBP2 and is mediated by UBE2I (PubMed:27160050).
Desumoylated by HINT1 (By similarity).