Rabbit Anti-RANGAP1 Recombinant Antibody (N-A) (CBMAB-R1293-CN)

Name: Rabbit Anti-RANGAP1 Recombinant Antibody (N-A)
SKU: CBMAB-R1293-CN
Rating: 5 (1 reviews)

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Datasheet

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Basic Information

Host Animal

Rabbit

Clone

N-A

Application

IHC-P, ICC, IF, WB

Immunogen

Synthetic peptide mixture: CHWSDMFTGRLRPEI (aa. 82-96) and CPSPEKLVRMGPRRSA (aa. 434-448) from Xenopus laevis

Specificity

Mouse, Human, Frog

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

More Infomation

Target

Full Name

Ran GTPase Activating Protein 1

Introduction

This gene encodes a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. The encoded protein interacts with Ras-related nuclear protein 1 (RAN) and regulates guanosine triphosphate (GTP)-binding and exchange. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Entrez Gene ID

Human	5905
Mouse	19387
Frog	100144714

UniProt ID

Human	P46060
Mouse	P46061
Frog	O13066

Alternative Names

Ran GTPase Activating Protein 1; SD; Segregation Distorter Homolog (Drosophila); Ran GTPase-Activating Protein 1; Segregation Distorter Homolog; Segregation Distortion;

Function

GTPase activator for RAN (PubMed:8146159, PubMed:8896452, PubMed:16428860).
Converts cytoplasmic GTP-bound RAN to GDP-bound RAN, which is essential for RAN-mediated nuclear import and export (PubMed:8896452, PubMed:27160050).
Mediates dissociation of cargo from nuclear export complexes containing XPO1, RAN and RANBP2 after nuclear export (PubMed:27160050).

Biological Process

Biological Process activation of GTPase activityManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process cellular response to vasopressinIEA:Ensembl
Biological Process negative regulation of protein export from nucleusManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process nuclear export1 PublicationIC:ComplexPortal
Biological Process nucleocytoplasmic transportManual Assertion Based On ExperimentIBA:GO_Central
Biological Process protein sumoylationManual Assertion Based On ExperimentIDA:ComplexPortal
Biological Process response to axon injuryIEA:Ensembl
Biological Process signal transductionManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Cytoplasm
Nucleus, nucleoplasm
Nucleus envelope
Chromosome, centromere, kinetochore
Cytoplasm, cytoskeleton, spindle
Cytoplasmic during interphase. Detected at the nuclear envelope during interphase (PubMed:11854305, PubMed:15037602).
Targeted to the nuclear pores after sumoylation (PubMed:11854305).
During mitosis, associates with mitotic spindles, but is essentially not detected at the spindle poles (PubMed:11854305, PubMed:15037602).
Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase (PubMed:11854305).
Mitotic location also requires sumoylation (PubMed:11854305).

PTM

Phosphorylation occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBE2I, on nuclear pore complex (NPC) diassembly and during mitosis.
Sumoylated (PubMed:11854305, PubMed:15037602, PubMed:26304119, PubMed:27160050).
Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis (PubMed:11854305).
Also required for interaction with RANBP2 and is mediated by UBE2I (PubMed:27160050).
Desumoylated by HINT1 (By similarity).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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