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Mouse Anti-RANGAP1 Recombinant Antibody (19C7) (CBMAB-R1288-CN)

This product is a Mouse antibody that recognizes RANGAP1. The antibody 19C7 can be used for immunoassay techniques such as: ELISA, FC, IF, WB.
See all RANGAP1 antibodies
Published Data
Fig.1 HFs were infected with wt HSV-1 (MOI 10) then samples harvested at 3, 6 and 10 h after infection were analyzed by western blot for the indicated proteins.
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Fig.2 Confocal microscopic images of mitotic HeLa cells transfected with control or RanBP2 siRNAs.
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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
19C7
Antibody Isotype
IgG1, κ
Application
ELISA, FC, IF, WB

Basic Information

Immunogen
A fusion protein consisting of the first 203 amino-terminal residues of the mouse RanGAP-1 protein
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Preservative
0.1% Sodium azide
Concentration
0.5 mg/mL

Target

Full Name
Ran GTPase Activating Protein 1
Introduction
This gene encodes a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. The encoded protein interacts with Ras-related nuclear protein 1 (RAN) and regulates guanosine triphosphate (GTP)-binding and exchange. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Entrez Gene ID
Human5905
Mouse19387
Rat362965
UniProt ID
HumanP46060
MouseP46061
RatQ66H32
Alternative Names
Ran GTPase Activating Protein 1; SD; Segregation Distorter Homolog (Drosophila); Ran GTPase-Activating Protein 1; Segregation Distorter Homolog; Segregation Distortion;
Function
GTPase activator for RAN (PubMed:8146159, PubMed:8896452, PubMed:16428860).
Converts cytoplasmic GTP-bound RAN to GDP-bound RAN, which is essential for RAN-mediated nuclear import and export (PubMed:8896452, PubMed:27160050).
Mediates dissociation of cargo from nuclear export complexes containing XPO1, RAN and RANBP2 after nuclear export (PubMed:27160050).
Biological Process
Biological Process activation of GTPase activityManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process cellular response to vasopressinIEA:Ensembl
Biological Process negative regulation of protein export from nucleusManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process nuclear export1 PublicationIC:ComplexPortal
Biological Process nucleocytoplasmic transportManual Assertion Based On ExperimentIBA:GO_Central
Biological Process protein sumoylationManual Assertion Based On ExperimentIDA:ComplexPortal
Biological Process response to axon injuryIEA:Ensembl
Biological Process signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Cytoplasm
Nucleus, nucleoplasm
Nucleus envelope
Chromosome, centromere, kinetochore
Cytoplasm, cytoskeleton, spindle
Cytoplasmic during interphase. Detected at the nuclear envelope during interphase (PubMed:11854305, PubMed:15037602).
Targeted to the nuclear pores after sumoylation (PubMed:11854305).
During mitosis, associates with mitotic spindles, but is essentially not detected at the spindle poles (PubMed:11854305, PubMed:15037602).
Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase (PubMed:11854305).
Mitotic location also requires sumoylation (PubMed:11854305).
PTM
Phosphorylation occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBE2I, on nuclear pore complex (NPC) diassembly and during mitosis.
Sumoylated (PubMed:11854305, PubMed:15037602, PubMed:26304119, PubMed:27160050).
Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis (PubMed:11854305).
Also required for interaction with RANBP2 and is mediated by UBE2I (PubMed:27160050).
Desumoylated by HINT1 (By similarity).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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