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Mouse Anti-RANGAP1 Recombinant Antibody (CBT3268) (V2LY-0625-LY216)


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Tested Data
Mouse Anti-RANGAP1 Recombinant Antibody (CBT3268) (V2LY-0625-LY216) in WB
Mouse Anti-RANGAP1 Recombinant Antibody (CBT3268) (V2LY-0625-LY216) in WB
Mouse Anti-RANGAP1 Recombinant Antibody (CBT3268) (V2LY-0625-LY216) in WB
Mouse Anti-RANGAP1 Recombinant Antibody (CBT3268) (V2LY-0625-LY216) in WB
Mouse Anti-RANGAP1 Recombinant Antibody (CBT3268) (V2LY-0625-LY216) in ELISA
Mouse Anti-RANGAP1 Recombinant Antibody (CBT3268) (V2LY-0625-LY216) in ELISA

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT3268
Antibody Isotype
IgG1
Application
WB

Basic Information

Immunogen
Purified recombinant fragment of human RANGAP1 (AA: 359-587) expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Ran GTPase Activating Protein 1
Entrez Gene ID
5905
UniProt ID
P46060
Function
GTPase activator for RAN (PubMed:8146159, PubMed:8896452, PubMed:16428860).
Converts cytoplasmic GTP-bound RAN to GDP-bound RAN, which is essential for RAN-mediated nuclear import and export (PubMed:8896452, PubMed:27160050).
Mediates dissociation of cargo from nuclear export complexes containing XPO1, RAN and RANBP2 after nuclear export (PubMed:27160050).
Biological Process
Biological Process activation of GTPase activityManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process cellular response to vasopressinIEA:Ensembl
Biological Process negative regulation of protein export from nucleusManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process nuclear export1 PublicationIC:ComplexPortal
Biological Process nucleocytoplasmic transportManual Assertion Based On ExperimentIBA:GO_Central
Biological Process protein sumoylationManual Assertion Based On ExperimentIDA:ComplexPortal
Biological Process response to axon injuryIEA:Ensembl
Biological Process signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Cytoplasm
Nucleus, nucleoplasm
Nucleus envelope
Chromosome, centromere, kinetochore
Cytoplasm, cytoskeleton, spindle
Cytoplasmic during interphase. Detected at the nuclear envelope during interphase (PubMed:11854305, PubMed:15037602).
Targeted to the nuclear pores after sumoylation (PubMed:11854305).
During mitosis, associates with mitotic spindles, but is essentially not detected at the spindle poles (PubMed:11854305, PubMed:15037602).
Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase (PubMed:11854305).
Mitotic location also requires sumoylation (PubMed:11854305).
PTM
Phosphorylation occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBE2I, on nuclear pore complex (NPC) diassembly and during mitosis.
Sumoylated (PubMed:11854305, PubMed:15037602, PubMed:26304119, PubMed:27160050).
Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis (PubMed:11854305).
Also required for interaction with RANBP2 and is mediated by UBE2I (PubMed:27160050).
Desumoylated by HINT1 (By similarity).
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For research use only. Not intended for any clinical use.

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