RECQL5

The protein encoded by this gene is a helicase that is important for genome stability. The encoded protein also prevents aberrant homologous recombination by displacing RAD51 from ssDNA. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]

Full Name

RecQ Like Helicase 5

Function

DNA helicase that plays an important role in DNA replication, transcription and repair (PubMed:20643585, PubMed:22973052).
Binds to the RNA polymerase II subunit POLR2A during transcription elongation and suppresses transcription-associated genomic instability (PubMed:20231364).
Associates also with POLR1A and enforces the stability of ribosomal DNA arrays (PubMed:27502483).
Plays an important role in mitotic chromosome separation after cross-over events and cell cycle progress (PubMed:22013166).
Mechanistically, removes RAD51 filaments protecting stalled replication forks at common fragile sites and stimulates MUS81-EME1 endonuclease leading to mitotic DNA synthesis (PubMed:28575661).
Required for efficient DNA repair, including repair of inter-strand cross-links (PubMed:23715498).
Stimulates DNA decatenation mediated by TOP2A. Prevents sister chromatid exchange and homologous recombination.

Biological Process

Biological Process cell divisionIEA:UniProtKB-KW
Biological Process cellular response to camptothecinIEA:Ensembl
Biological Process cellular response to ketoneIEA:Ensembl
Biological Process cellular response to xenobiotic stimulusIEA:Ensembl
Biological Process chromosome separationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process DNA duplex unwindingManual Assertion Based On ExperimentIBA:GO_Central
Biological Process DNA metabolic process1 PublicationNAS:UniProtKB
Biological Process DNA recombinationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process DNA repairManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process DNA replicationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process DNA unwinding involved in DNA replicationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process double-strand break repair via homologous recombinationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process mitotic cell cycleManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process negative regulation of double-strand break repair via homologous recombinationIEA:Ensembl
Biological Process negative regulation of transcription elongation from RNA polymerase II promoterManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process replication-born double-strand break repair via sister chromatid exchangeIEA:Ensembl

Cellular Location

Isoform Beta
Nucleus, nucleoplasm
Nucleus
Recruited to sites of DNA damage, such as single-strand breaks and inter-strand cross-links, and at stalled replication forks. Re-localizes from the nucleolus into the nucleus after replicative stress and significantly associates with WRN during S-phase (PubMed:23180761).
Isoform Alpha
Cytoplasm
Isoform Gamma
Cytoplasm

PTM

Phosphorylated by CDK1 at Ser-727; this phosphorylation is required for RECQL5-mediated disruption of RAD51 filaments on stalled replication forks.