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Mouse Th1/Th2/Th17 Antibody Array (Glass Slide, 18 Targets) (Y0119) (AbAr-0119-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Mouse Th1/Th2/Th17 Antibody Array detects 18 Mouse Th1, Th2, and Th17 Cytokines. It is suitable for all liquid sample types.
See all Th1/Th2/Th17 antibodies

Specifications

Target Array
Th1/Th2/Th17 Array
Target
IFN-gamma, IL-17A, IL-23 p19, MIP-3 alpha (CCL20), IL-1 beta (IL-1 F2), IL-17F, IL-28A (IFN-lambda 2), TGF beta 1, IL-10, IL-2, IL-4, TNF alpha, IL-12 p70, IL-21, IL-5, IL-13, IL-22, IL-6
Species Reactivity
Mouse
Sample Type
Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates, Other Body Fluids

Applications

Application
Multiplex Protein Detection
Assay Type
Quantitative
Solid Support
Glass Slide
Detection Assay
Sandwich-based
Protocol Outline
Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis
Components
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction
CD4 T-cells can be subdivided into Th1, Th2, Th17, and Treg subsets on the basis of their pattern of cytokine production. Protective Th1-related cytokines (Interleukin (IL)-2 and interferon (IFN)-γ) are involved in cellular immune responses. Th2-related cytokines (IL-4 and IL-10) are associated with humoral immunity and anti-inflammatory properties. However, these arise in a later period of periodontitis and are involved in the chronic progression of the disease. Th17 was identified as cells that recruited neutrophils and macrophages to participate and amplify the inflammatory reaction.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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