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Human Th1/Th2/Th17 Antibody Array (Glass Slide, 27 Targets) (Y0337) (AbAr-0337-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Human Th1/Th2/Th17 Antibody Array can be used for quantitative measurement of 27 Human cytokines and receptors. It is suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.
See all Th1/Th2/Th17 antibodies

Specifications

Target Array
Th1/Th2/Th17 Array
Target
CD40 (TNFRSF5), CD40 Ligand (TNFSF5), CTLA-4 (CD152), G-CSF, GITR (TNFRSF18), IL-1 R1, IL-1 R2, IL-1 R3 (IL-1 R Acp), IL-1 R4 (ST2), IL-1 R6 (IL-1 Rrp2), IL-6 R, IL-9, IL-12 p40, IL-31, LAG-3, LAP/TGF beta 1, L-Selectin (CD62L), MCP-3 (MARC/CCL7), MIP-1 alpha (CCL3), NCAM-1 (CD56), PD-1, PDGF R alpha, PDGF R beta, SCF, TGF beta 2, TIM-1 (KIM-1), TRANCE (TNFSF11)
Species Reactivity
Human
Sample Type
Serum, Plasma, Other Biological Fluids, Cell Lysates, Cell Culture Media, Tissue Lysates

Applications

Application
Multiplex Protein Detection
Assay Type
Quantitative
Solid Support
Glass Slide
Detection Assay
Label-based
Protocol Outline
Dry the glass slide
Incubate samples
Incubate with biotin lectin mixture
Incubate with Cy3 Equivalent Dye-Streptavidin
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis
Components
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction
CD4 T-cells can be subdivided into Th1, Th2, Th17, and Treg subsets on the basis of their pattern of cytokine production. Protective Th1-related cytokines (Interleukin (IL)-2 and interferon (IFN)-γ) are involved in cellular immune responses. Th2-related cytokines (IL-4 and IL-10) are associated with humoral immunity and anti-inflammatory properties. However, these arise in a later period of periodontitis and are involved in the chronic progression of the disease. Th17 was identified as cells that recruited neutrophils and macrophages to participate and amplify the inflammatory reaction.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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