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Human AKI Antibody Array (Membrane, 20 Targets) (Y0002) (AbAr-0002-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Human AKI Antibody Array detects 20 Human Cytokines. It is suitable for all liquid sample types.
See all AKI antibodies

Specifications

Target Array
AKI Array
Target
TIM-1(KIM-1), Albumin, Osteopontin(SPP1), TFF3(Trefoil Factor 3), Beta-2 Microglobulin, Clusterin, CXCL16, Osteoactivin(GPNMB), FABP1, MCP-1(CCL2), TNF RI(TNFRSF1A), Calbindin, IP-10(CXCL10), Cystatin C, HGF, MIF, Lipocalin-2(NGAL), TIMP-1, VCAM-1(CD106), VEGF-A
Species Reactivity
Human
Sample Type
Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates

Applications

Application
Multiplex Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Membrane
Detection Assay
Sandwich-based
Protocol Outline
Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction
Serum cytokines are increased in patients with acute kidney injury (AKI), and increased serum interleukin (IL)-6, IL-8 and IL-10 predict mortality in patients with AKI. Cytokines are not just biomarkers of poor outcomes, but mediate deleterious systemic effects. Pro-inflammatory cytokines such as IL-6 and IL-8 mediate distant organ injury and contribute to the systemic inflammatory response syndrome and acute lung injury (ALI). Anti-inflammatory cytokines such as IL-10 may contribute to immune stasis and impair the ability to fight infection. These cytokine-mediated deleterious effects may contribute to the high mortality of patients with AKI. Thus, a better understanding of the production and clearance of cytokines in AKI may shed light onto potential therapeutic treatments for patients with AKI.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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