Rat AKI Antibody Array (Glass Slide, 7 Targets) (Y0144) (AbAr-0144-YC)

Name: Rat AKI Antibody Array (Glass Slide, 7 Targets) (Y0144)
SKU: AbAr-0144-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Specifications

Target Array

AKI Array

Target

TIM-1, TIMP-1, FABP1, VEGF-A, CCL2, Cystatin C, Lipocalin-2

Species Reactivity

Rat

Sample Type

Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates

Applications

Application

Multiplex Protein Detection

Assay Type

Semi-Quantitative

Solid Support

Glass Slide

Detection Assay

Sandwich-based

Protocol Outline

Thoroughly dry the glass slide.
Block the surface of the array.
Incubate with the sample.
Incubate using a biotinylated detection antibody cocktail and then with streptavidin-conjugated fluorescent dye.
Carefully disassemble the glass slide and scan using a gene microarray laser scanner.
Conduct densitometric analysis.

Components

Antibody Array Slide
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction

Serum cytokines are increased in patients with acute kidney injury (AKI), and increased serum interleukin (IL)-6, IL-8 and IL-10 predict mortality in patients with AKI. Cytokines are not just biomarkers of poor outcomes, but mediate deleterious systemic effects. Pro-inflammatory cytokines such as IL-6 and IL-8 mediate distant organ injury and contribute to the systemic inflammatory response syndrome and acute lung injury (ALI). Anti-inflammatory cytokines such as IL-10 may contribute to immune stasis and impair the ability to fight infection. These cytokine-mediated deleterious effects may contribute to the high mortality of patients with AKI. Thus, a better understanding of the production and clearance of cytokines in AKI may shed light onto potential therapeutic treatments for patients with AKI.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human AKI Antibody Array (Membrane, 20 Targets) (Y0002) (CAT#: AbAr-0002-YC)

Rat AKI Antibody Array (Membrane, 7 Targets) (Y0142) (CAT#: AbAr-0142-YC)

Human AKI Antibody Array (Glass Slide, 20 Targets) (Y0010) (CAT#: AbAr-0010-YC)

Antibody Array

Human AKI Antibody Array (Membrane, 20 Targets) (Y0002) (CAT#: AbAr-0002-YC)

Rat AKI Antibody Array (Membrane, 7 Targets) (Y0142) (CAT#: AbAr-0142-YC)

Human AKI Antibody Array (Glass Slide, 20 Targets) (Y0010) (CAT#: AbAr-0010-YC)

Related Products

Human AKI Antibody Array (Glass Slide, 20 Targets) (Y0010) (CAT#: AbAr-0010-YC)

Rat AKI Antibody Array (Membrane, 7 Targets) (Y0142) (CAT#: AbAr-0142-YC)

Human AKI Antibody Array (Membrane, 20 Targets) (Y0002) (CAT#: AbAr-0002-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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