TIM-1 (KIM-1), TIMP-1, FABP1, VEGF-A, MCP-1 (CCL2), Cystatin C, Lipocalin-2 (NGAL)
Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates
Multiplex Protein Detection
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
Antibody Array Membrane
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.
Serum cytokines are increased in patients with acute kidney injury (AKI), and increased serum interleukin (IL)-6, IL-8 and IL-10 predict mortality in patients with AKI. Cytokines are not just biomarkers of poor outcomes, but mediate deleterious systemic effects. Pro-inflammatory cytokines such as IL-6 and IL-8 mediate distant organ injury and contribute to the systemic inflammatory response syndrome and acute lung injury (ALI). Anti-inflammatory cytokines such as IL-10 may contribute to immune stasis and impair the ability to fight infection. These cytokine-mediated deleterious effects may contribute to the high mortality of patients with AKI. Thus, a better understanding of the production and clearance of cytokines in AKI may shed light onto potential therapeutic treatments for patients with AKI.