What applications has the Rabbit Anti-A2M Recombinant Antibody (CBMAB-A0030-YC) been validated for?

This recombinant antibody has been validated for use in various applications including Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), and ELISA. Our internal validation ensures high specificity and sensitivity in these commonly used experimental procedures.

What species reactivity does this Rabbit Anti-A2M Recombinant Antibody (CBMAB-A0030-YC) have?

This antibody is designed to react with Human A2M. While we do not guarantee reactivity with other species unless explicitly stated on the datasheet, sequence homology may allow for cross-reactivity in certain related species. We recommend checking the provided datasheet for the most up-to-date information on tested species reactivity.

Rabbit Anti-A2M Recombinant Antibody (V2-178817) (CBMAB-A0030-YC)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

V2-178817

Application

ELISA

Immunogen

Recombinant Human alpha-2-macroglobulin Protein

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:5,000-1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS

Preservative

None

Concentration

1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Alpha-2-Macroglobulin

Introduction

A2M is a protease inhibitor and cytokine transporter. It uses a bait-and-trap mechanism to inhibit a broad spectrum of proteases, including trypsin, thrombin and collagenase. It can also inhibit inflammatory cytokines, and it thus disrupts inflammatory ca

Entrez Gene ID

UniProt ID

P01023

Alternative Names

Alpha-2-Macroglobulin; C3 And PZP-Like Alpha-2-Macroglobulin Domain-Containing Protein 5; Alpha-2-M; CPAMD5; FWP007; S863-7; A2MD;

Function

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Biological Process

Extracellular matrix disassembly
Negative regulation of blood coagulation, intrinsic pathway
Negative regulation of complement activation, lectin pathway
Platelet degranulation
Regulation of small GTPase mediated signal transduction
Stem cell differentiation

Cellular Location

Secreted

Harwood, S. L., Lyngsø, J., Zarantonello, A., Kjøge, K., Nielsen, P. K., Andersen, G. R., ... & Enghild, J. J. (2021). Structural investigations of human A2M identify a hollow native conformation that underlies its distinctive protease-trapping mechanism. Molecular & Cellular Proteomics, 20.

Cattaneo, A., Cattane, N., Malpighi, C., Czamara, D., Suarez, A., Mariani, N., ... & Pariante, C. M. (2018). FoxO1, A2M, and TGF-β1: three novel genes predicting depression in gene X environment interactions are identified using cross-species and cross-tissues transcriptomic and miRNomic analyses. Molecular psychiatry, 23(11), 2192-2208.

Zhang, Y., Wei, X., Browning, S., Scuderi, G., Hanna, L. S., & Wei, L. (2017). Targeted designed variants of alpha-2-macroglobulin (A2M) attenuate cartilage degeneration in a rat model of osteoarthritis induced by anterior cruciate ligament transection. Arthritis research & therapy, 19(1), 1-11.

Harwood, S. L., Nielsen, N. S., Pedersen, H., Kjøge, K., Nielsen, P. K., Andersen, G. R., & Enghild, J. J. (2020). Substituting the Thiol Ester of Human A2M or C3 with a Disulfide Produces Native Proteins with Altered Proteolysis-Induced Conformational Changes. Biochemistry, 59(51), 4799-4809.

Kurz, S., Thieme, R., Amberg, R., Groth, M., Jahnke, H. G., Pieroh, P., ... & Birkenmeier, G. (2017). The anti-tumorigenic activity of A2M—A lesson from the naked mole-rat. PloS one, 12(12), e0189514.

Zhao, M., Chen, L., Qiao, Z., Zhou, J., Zhang, T., Zhang, W., ... & Yang, X. (2020). Association between FoxO1, A2M, and TGF-β1, environmental factors, and major depressive disorder. Frontiers in psychiatry, 11, 675.

Mohindra, V., Dangi, T., Chowdhury, L. M., & Jena, J. K. (2019). Tissue specific alpha-2-Macroglobulin (A2M) splice isoform diversity in Hilsa shad, Tenualosa ilisha (Hamilton, 1822). Plos one, 14(7), e0216144.

Pan, X. Y., & Zhang, Z. W. (2020). MFGE8, ALB, APOB, APOE, SAA1, A2M, and C3 as novel biomarkers for stress cardiomyopathy. Cardiovascular therapeutics, 2020.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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