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Rabbit Anti-A2M Recombinant Antibody (V2-178817) (CBMAB-A0030-YC)

Provided herein is a Rabbit monoclonal antibody against Human Alpha-2-Macroglobulin. The antibody can be used for immunoassay techniques, such as ELISA.
See all A2M antibodies

Summary

Host Animal
Rabbit
Specificity
Human
Clone
V2-178817
Antibody Isotype
IgG
Application
ELISA

Basic Information

Immunogen
Recombinant Human alpha-2-macroglobulin Protein
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
ELISA1:5,000-1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS
Preservative
None
Concentration
1 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Alpha-2-Macroglobulin
Introduction
A2M is a protease inhibitor and cytokine transporter. It uses a bait-and-trap mechanism to inhibit a broad spectrum of proteases, including trypsin, thrombin and collagenase. It can also inhibit inflammatory cytokines, and it thus disrupts inflammatory ca
Entrez Gene ID
UniProt ID
Alternative Names
Alpha-2-Macroglobulin; C3 And PZP-Like Alpha-2-Macroglobulin Domain-Containing Protein 5; Alpha-2-M; CPAMD5; FWP007; S863-7; A2MD;
Function
Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
Biological Process
Extracellular matrix disassembly
Negative regulation of blood coagulation, intrinsic pathway
Negative regulation of complement activation, lectin pathway
Platelet degranulation
Regulation of small GTPase mediated signal transduction
Stem cell differentiation
Cellular Location
Secreted

Harwood, S. L., Lyngsø, J., Zarantonello, A., Kjøge, K., Nielsen, P. K., Andersen, G. R., ... & Enghild, J. J. (2021). Structural investigations of human A2M identify a hollow native conformation that underlies its distinctive protease-trapping mechanism. Molecular & Cellular Proteomics, 20.

Cattaneo, A., Cattane, N., Malpighi, C., Czamara, D., Suarez, A., Mariani, N., ... & Pariante, C. M. (2018). FoxO1, A2M, and TGF-β1: three novel genes predicting depression in gene X environment interactions are identified using cross-species and cross-tissues transcriptomic and miRNomic analyses. Molecular psychiatry, 23(11), 2192-2208.

Zhang, Y., Wei, X., Browning, S., Scuderi, G., Hanna, L. S., & Wei, L. (2017). Targeted designed variants of alpha-2-macroglobulin (A2M) attenuate cartilage degeneration in a rat model of osteoarthritis induced by anterior cruciate ligament transection. Arthritis research & therapy, 19(1), 1-11.

Harwood, S. L., Nielsen, N. S., Pedersen, H., Kjøge, K., Nielsen, P. K., Andersen, G. R., & Enghild, J. J. (2020). Substituting the Thiol Ester of Human A2M or C3 with a Disulfide Produces Native Proteins with Altered Proteolysis-Induced Conformational Changes. Biochemistry, 59(51), 4799-4809.

Kurz, S., Thieme, R., Amberg, R., Groth, M., Jahnke, H. G., Pieroh, P., ... & Birkenmeier, G. (2017). The anti-tumorigenic activity of A2M—A lesson from the naked mole-rat. PloS one, 12(12), e0189514.

Zhao, M., Chen, L., Qiao, Z., Zhou, J., Zhang, T., Zhang, W., ... & Yang, X. (2020). Association between FoxO1, A2M, and TGF-β1, environmental factors, and major depressive disorder. Frontiers in psychiatry, 11, 675.

Mohindra, V., Dangi, T., Chowdhury, L. M., & Jena, J. K. (2019). Tissue specific alpha-2-Macroglobulin (A2M) splice isoform diversity in Hilsa shad, Tenualosa ilisha (Hamilton, 1822). Plos one, 14(7), e0216144.

Pan, X. Y., & Zhang, Z. W. (2020). MFGE8, ALB, APOB, APOE, SAA1, A2M, and C3 as novel biomarkers for stress cardiomyopathy. Cardiovascular therapeutics, 2020.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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