Rat A2m ELISA Kit (2) (V2LY-0626-LY2)

Name: Rat A2m ELISA Kit (2)
SKU: V2LY-0626-LY2
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.24 mg/mL

Detection Range

0.5-100 mg/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Rat

Assay Type

Sandwich

Reactivity

Rat

Assay Time

1.5 h

Molecule Mass

163.8 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Alpha-2-Macroglobulin

Function

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Biological Process

Extracellular matrix disassembly
Negative regulation of blood coagulation, intrinsic pathway
Negative regulation of complement activation, lectin pathway
Platelet degranulation
Regulation of small GTPase mediated signal transduction
Stem cell differentiation

Cellular Location

Secreted

Harwood, S. L., Lyngsø, J., Zarantonello, A., Kjøge, K., Nielsen, P. K., Andersen, G. R., ... & Enghild, J. J. (2021). Structural investigations of human A2M identify a hollow native conformation that underlies its distinctive protease-trapping mechanism. Molecular & Cellular Proteomics, 20.

Cattaneo, A., Cattane, N., Malpighi, C., Czamara, D., Suarez, A., Mariani, N., ... & Pariante, C. M. (2018). FoxO1, A2M, and TGF-β1: three novel genes predicting depression in gene X environment interactions are identified using cross-species and cross-tissues transcriptomic and miRNomic analyses. Molecular psychiatry, 23(11), 2192-2208.

Zhang, Y., Wei, X., Browning, S., Scuderi, G., Hanna, L. S., & Wei, L. (2017). Targeted designed variants of alpha-2-macroglobulin (A2M) attenuate cartilage degeneration in a rat model of osteoarthritis induced by anterior cruciate ligament transection. Arthritis research & therapy, 19(1), 1-11.

Harwood, S. L., Nielsen, N. S., Pedersen, H., Kjøge, K., Nielsen, P. K., Andersen, G. R., & Enghild, J. J. (2020). Substituting the Thiol Ester of Human A2M or C3 with a Disulfide Produces Native Proteins with Altered Proteolysis-Induced Conformational Changes. Biochemistry, 59(51), 4799-4809.

Kurz, S., Thieme, R., Amberg, R., Groth, M., Jahnke, H. G., Pieroh, P., ... & Birkenmeier, G. (2017). The anti-tumorigenic activity of A2M—A lesson from the naked mole-rat. PloS one, 12(12), e0189514.

Zhao, M., Chen, L., Qiao, Z., Zhou, J., Zhang, T., Zhang, W., ... & Yang, X. (2020). Association between FoxO1, A2M, and TGF-β1, environmental factors, and major depressive disorder. Frontiers in psychiatry, 11, 675.

Mohindra, V., Dangi, T., Chowdhury, L. M., & Jena, J. K. (2019). Tissue specific alpha-2-Macroglobulin (A2M) splice isoform diversity in Hilsa shad, Tenualosa ilisha (Hamilton, 1822). Plos one, 14(7), e0216144.

Pan, X. Y., & Zhang, Z. W. (2020). MFGE8, ALB, APOB, APOE, SAA1, A2M, and C3 as novel biomarkers for stress cardiomyopathy. Cardiovascular therapeutics, 2020.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant A2M protein, His Tag (CAT#: V2LY-0526-LY2051)

Rat A2m ELISA Kit (CAT#: V2LY-0626-LY1948)

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Rat A2m ELISA Kit (CAT#: V2LY-0626-LY1948)

Human Recombinant A2M protein, His Tag (CAT#: V2LY-0526-LY2051)

Rabbit Anti-A2M Recombinant Antibody (V2-178837) (CAT#: CBMAB-A0052-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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