What applications is the Mouse Anti-ABAT Recombinant Antibody (V2-178880) validated for?

This recombinant antibody (CAT: CBMAB-A0107-YC) is validated for Western Blot (WB), Immunofluorescence (IF), and ELISA. While it has performed excellently in these applications, we recommend referring to the product datasheet on the Creative Biolabs website for the most up-to-date and complete list of validated applications and recommended dilutions.

Is this antibody suitable for use with human or other species' samples, given it's a "Mouse Anti-ABAT" antibody?

The "Mouse Anti-ABAT" indicates that the antibody was raised in a mouse against the ABAT antigen. The product datasheet for CBMAB-A0107-YC specifies its reactivity with human ABAT. While sequence homology can sometimes predict cross-reactivity, we always recommend checking the product's specific "Species Reactivity" section on the Creative Biolabs website or datasheet. For untested species, a small trial size might be available for evaluation.

Mouse Anti-ABAT Recombinant Antibody (V2-178880) (CBMAB-A0107-YC)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-178880

Application

IHC, WB

Immunogen

Human recombinant protein fragment corresponding to amino acids 29-323 of human ABAT(NP_065737) produced in E. coli

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:2,000
IHC	1:150

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH7.3, 1% BSA, 50% Glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

4-Aminobutyrate Aminotransferase

Introduction

4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD

Entrez Gene ID

UniProt ID

P80404

Alternative Names

4-Aminobutyrate Aminotransferase; (S)-3-Amino-2-Methylpropionate Transaminase; Gamma-Amino-N-Butyrate Transaminase; 4-Aminobutyrate Transaminase; GABA Aminotransferase; GABA Transaminase; GABA-AT; GABAT;

Function

Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine.

Biological Process

Behavioral response to cocaine
Gamma-aminobutyric acid catabolic process
Gamma-aminobutyric acid metabolic process
Neurotransmitter catabolic process

Cellular Location

Mitochondrion matrix

Involvement in disease

An enzymatic deficiency resulting in psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities.

Zhao, G., Li, N., Li, S., Wu, W., Wang, X., & Gu, J. (2019). High methylation of the 4-aminobutyrate aminotransferase gene predicts a poor prognosis in patients with myelodysplastic syndrome. International journal of oncology, 54(2), 491-504.

Shen, J., Wang, C., Ying, J., Xu, T., McAlinden, A., & O’Keefe, R. J. (2019). Inhibition of 4-aminobutyrate aminotransferase protects against injury-induced osteoarthritis in mice. JCI insight, 4(18).

Chen, Y., Zhao, G., Li, N., Luo, Z., Wang, X., & Gu, J. (2019). Role of 4‑aminobutyrate aminotransferase (ABAT) and the lncRNA co‑expression network in the development of myelodysplastic syndrome. Oncology reports, 42(2), 509-520.

Shen, J., Wang, C., Li, D., Wang, T., McAlinden, A., & O'Keefe, R. (2018). Methylation of 4-aminobutyrate aminotransferase (ABAT) by dnmy3b regulates chondrocytes metabolism and the development of osteoarthritis (OA). Osteoarthritis and Cartilage, 26, S64-S65.

Nagappa, M., Bindu, P. S., Chiplunkar, S., Govindaraj, P., Narayanappa, G., Krishnan, A., ... & Taly, A. B. (2017). Hypersomnolence-hyperkinetic movement disorder in a child with compound heterozygous mutation in 4-aminobutyrate aminotransferase (ABAT) gene. Brain and Development, 39(2), 161-165.

Iftikhar, H., Batool, S., Deep, A., Narasimhan, B., Sharma, P. C., & Malhotra, M. (2017). In silico analysis of the inhibitory activities of GABA derivatives on 4-aminobutyrate transaminase. Arabian Journal of Chemistry, 10, S1267-S1275.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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