Mouse Anti-ABAT Recombinant Antibody (V2-178890) (CBMAB-A0117-YC)

Provided herein is a Mouse monoclonal antibody against Human 4-Aminobutyrate Aminotransferase. The antibody can be used for immunoassay techniques, such as IHC.
See all ABAT antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

V2-178890

Antibody Isotype

IgG2a

Application

IHC

Basic Information

Immunogen

Specific for an epitope mapping between amino acids 357-391 within an internal region of ABAT of human origin

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
ELISA	1:30-1:3,000
IF(ICC)	1:50-1:500
IP	1-2 µl per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

4-Aminobutyrate Aminotransferase

Introduction

4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD

Entrez Gene ID

UniProt ID

P80404

Alternative Names

4-Aminobutyrate Aminotransferase; (S)-3-Amino-2-Methylpropionate Transaminase; Gamma-Amino-N-Butyrate Transaminase; 4-Aminobutyrate Transaminase; GABA Aminotransferase; GABA Transaminase; GABA-AT; GABAT;

Function

Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine.

Biological Process

Behavioral response to cocaine
Gamma-aminobutyric acid catabolic process
Gamma-aminobutyric acid metabolic process
Neurotransmitter catabolic process

Cellular Location

Mitochondrion matrix

Involvement in disease

An enzymatic deficiency resulting in psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities.

References

Zhao, G., Li, N., Li, S., Wu, W., Wang, X., & Gu, J. (2019). High methylation of the 4-aminobutyrate aminotransferase gene predicts a poor prognosis in patients with myelodysplastic syndrome. International journal of oncology, 54(2), 491-504.

Shen, J., Wang, C., Ying, J., Xu, T., McAlinden, A., & O’Keefe, R. J. (2019). Inhibition of 4-aminobutyrate aminotransferase protects against injury-induced osteoarthritis in mice. JCI insight, 4(18).

Chen, Y., Zhao, G., Li, N., Luo, Z., Wang, X., & Gu, J. (2019). Role of 4‑aminobutyrate aminotransferase (ABAT) and the lncRNA co‑expression network in the development of myelodysplastic syndrome. Oncology reports, 42(2), 509-520.

Shen, J., Wang, C., Li, D., Wang, T., McAlinden, A., & O'Keefe, R. (2018). Methylation of 4-aminobutyrate aminotransferase (ABAT) by dnmy3b regulates chondrocytes metabolism and the development of osteoarthritis (OA). Osteoarthritis and Cartilage, 26, S64-S65.

Nagappa, M., Bindu, P. S., Chiplunkar, S., Govindaraj, P., Narayanappa, G., Krishnan, A., ... & Taly, A. B. (2017). Hypersomnolence-hyperkinetic movement disorder in a child with compound heterozygous mutation in 4-aminobutyrate aminotransferase (ABAT) gene. Brain and Development, 39(2), 161-165.

Iftikhar, H., Batool, S., Deep, A., Narasimhan, B., Sharma, P. C., & Malhotra, M. (2017). In silico analysis of the inhibitory activities of GABA derivatives on 4-aminobutyrate transaminase. Arabian Journal of Chemistry, 10, S1267-S1275.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

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Mouse Anti-ABAT Recombinant Antibody (V2-178886)

Mouse Anti-ABAT Recombinant Antibody (V2-178888)

Mouse Anti-ABAT Recombinant Antibody (V2-178889)

Mouse Anti-ABAT Recombinant Antibody (V2-178880)

Mouse Anti-ABAT Recombinant Antibody (V2-178883)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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