ELISA, FC, IF, IHC-P, WB
The AB.H10 antibody has been reported tested in various applications including WB, IP, IF, IHC, FCM.
For western blot, the recommended concentration is 1:200-1:500. The theoretical molecular weight is 254KDa. The recommended blocking agent is 4% BSA and the recommended total protein is 80-100µg.
For IF and IHC, the recommended dilution is 1:200. Before IHC staining, a kindly reminder that please conduct heat mediated antigen retrieval before commencing.
For flow cytometry, the recommended reported PBS buffer contains 5% FBS, and incubation time is 30 min at 4°C. The recommended concentration is 1-2 µg for 10^6 cells.
For ELISA, the concentration is dependent on the specific assay.
It's recommended that the optimal antibody concentration, dilution, incubation time etc. are best to be carefully titrated and determined by the end user.
Human ABCA1 aa 1800-2260
Mouse, Rat, Chicken, Bovine, Human
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White).The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. cAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport. Mutations in this gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency.
ATP-Binding Cassette, Sub-Family A (ABC1), Member 1; Cholesterol Efflux Regulatory Protein; ABC-1; ABC1; CERP; ATP-Binding Cassette Transporter A1; ATP-Binding Cassette Transporter 1; ATP-Binding Cassette 1; Tangier Disease; Membrane-Bound; EC 22.214.171.124; EC 126.96.36.199; EC 188.8.131.52; EC 3.6.3; HDLDT1; TGD