Mouse Anti-ABCA1 Recombinant Antibody (V2-6057) (CBMAB-0002CQ)

Name: Mouse Anti-ABCA1 Recombinant Antibody (V2-6057)
SKU: CBMAB-0002CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-6057

Application

ELISA, FC, IF, IHC-P, WB

Immunogen

Human ABCA1 aa 1800-2260

Specificity

Human, Mouse, Rat, Chicken, Cattle

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The AB.H10 antibody has been reported tested in various applications including WB, IP, IF, IHC, FCM.
For western blot, the recommended concentration is 1:200-1:500. The theoretical molecular weight is 254KDa. The recommended blocking agent is 4% BSA and the recommended total protein is 80-100µg.
For IF and IHC, the recommended dilution is 1:200. Before IHC staining, a kindly reminder that please conduct heat mediated antigen retrieval before commencing.
For flow cytometry, the recommended reported PBS buffer contains 5% FBS, and incubation time is 30 min at 4°C. The recommended concentration is 1-2 µg for 10^6 cells.
For ELISA, the concentration is dependent on the specific assay.
It's recommended that the optimal antibody concentration, dilution, incubation time etc. are best to be carefully titrated and determined by the end user.
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:200-1:500
IHC	1:200
FC	1-2 µg/10^6 cells

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.2

Preservative

0.05% sodium azide

Concentration

Batch dependent

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ATP Binding Cassette Subfamily A Member 1

Introduction

ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White).The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. cAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport. Mutations in this gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency.

Entrez Gene ID

Human	19
Mouse	11303
Rat	313210
Chicken	373945
Cattle	535379

UniProt ID

Human	O95477
Mouse	P41233
Rat	F1LNL3
Cattle	F1MG24

Alternative Names

ATP-Binding Cassette, Sub-Family A (ABC1), Member 1; Cholesterol Efflux Regulatory Protein; ABC-1; ABC1; CERP; ATP-Binding Cassette Transporter A1; ATP-Binding Cassette Transporter 1; ATP-Binding Cassette 1; Tangier Disease; Membrane-Bound; EC 3.6.3.41; EC 2.8.1.8; EC 2.7.7.8; EC 3.6.3; HDLDT1; TGD

Function

Catalyzes the translocation of specific phospholipids from the cytoplasmic to the extracellular/lumenal leaflet of membrane coupled to the hydrolysis of ATP. Thereby, participates in phospholipid transfer to apoliproteins to form nascent high density lipoproteins/HDLs. Transports preferentially phosphatidylcholine over phosphatidylserine. May play a similar role in the efflux of intracellular cholesterol to apoliproteins and the formation of nascent high density lipoproteins/HDLs

Biological Process

Adenylate cyclase-activating G protein-coupled receptor signaling pathway
Cellular response to low-density lipoprotein particle stimulus
Cholesterol efflux
Cholesterol homeostasis
Cholesterol metabolic process
Endosomal transport
Export across plasma membrane
G protein-coupled receptor signaling pathway
High-density lipoprotein particle assembly
Intracellular cholesterol transport
Intracellular receptor signaling pathway
Lipid transport
Lysosome organization
Negative regulation of cholesterol storage
Negative regulation of macrophage derived foam cell differentiation
Phospholipid efflux
Phospholipid homeostasis
Phospholipid translocation
Platelet dense granule organization
Positive regulation of cholesterol efflux
Positive regulation of high-density lipoprotein particle assembly
Protein secretion
Protein transmembrane transport
Regulation of Cdc42 protein signal transduction
Regulation of high-density lipoprotein particle assembly
Regulation of metabolic process
Response to laminar fluid shear stress
Reverse cholesterol transport
Signal release

Cellular Location

Endosome; Cell membrane

Involvement in disease

An autosomal recessive disorder characterized by near absence of plasma high density lipoproteins, low serum HDL cholesterol, and massive tissue deposition of cholesterol esters. Clinical features include large yellow-orange tonsils, hepatomegaly, splenomegaly, enlarged lymph nodes, and often sensory polyneuropathy.
An autosomal dominant disorder characterized by decreased plasma high density lipoproteins, moderately low HDL cholesterol, a reduction in cellular cholesterol efflux, and susceptibility to premature coronary artery disease.

Topology

Helical: 22-42 aa
Extracellular: 43-639 aa
Helical: 640-1371 aa
Extracellular: 1372-1656 aa
Helical: 1657-1872 aa

PTM

Phosphorylation on Ser-2054 regulates phospholipid efflux.
Palmitoylated by ZDHHC8. Palmitoylation is essential for localization to the plasma membrane

Wang, S. M., Lin, W. C., Lin, H. Y., Chen, Y. L., Ko, C. Y., & Wang, J. M. (2021). CCAAT/Enhancer-binding protein delta mediates glioma stem-like cell enrichment and ATP-binding cassette transporter ABCA1 activation for temozolomide resistance in glioblastoma. Cell Death Discovery, 7(1), 1-11.

Li, J., Wen, M., Zhang, Z., Qiu, Z., & Sun, Y. (2020). The R219K polymorphism of the ATP binding cassette subfamily A member 1 gene and susceptibility to ischemic stroke in Chinese population. Open Medicine, 15(1), 274-282.

Jiang, P., Zhao, Z., Li, X., Wang, M., Xia, L., Cao, Y., ... & Fang, X. (2020). RNA interference mediated knockdown of ATP binding cassette subfamily A member 1 decreases the triglyceride content of bovine mammary epithelial cells. Pakistan Journal of Zoology, 52(1), 239.

Chen, Z., Chu, S., Wang, X., Fan, Y., Zhan, T., Arbab, A. A. I., ... & Yang, Z. (2019). MicroRNA-106b regulates milk fat metabolism via ATP binding cassette subfamily A member 1 (ABCA1) in bovine mammary epithelial cells. Journal of agricultural and food chemistry, 67(14), 3981-3990.

Yan, H., Cheng, L., Jia, R., Yao, H., Wu, H., Shen, Y., ... & Zhang, Z. (2019). ATP-binding cassette sub-family a member1 gene mutation improves lipid metabolic abnormalities in diabetes mellitus. Lipids in health and disease, 18(1), 1-6.

Hou, L., Tang, S., Wu, B. J., Ong, K. L., Westerterp, M., Barter, P. J., ... & Rye, K. A. (2019). Apolipoprotein A‐l improves pancreatic β‐cell function independent of the ATP‐binding cassette transporters ABCA1 and ABCG1. The FASEB Journal, 33(7), 8479-8489.

Jung, D., Cao, S., Liu, M., & Park, S. (2018). A meta-analysis of the associations between the ATP-binding cassette transporter ABCA1 R219K (rs2230806) polymorphism and the risk of type 2 diabetes in Asians. Hormone and Metabolic Research, 50(04), 308-316.

Wang, L., Fumoto, T., Masumoto, S., Shoji, T., Miura, T., Naraoka, M., ... & Ohkuma, H. (2017). Regression of atherosclerosis with apple procyanidins by activating the ATP-binding cassette subfamily A member 1 in a rabbit model. Atherosclerosis, 258, 56-64.

Hafiane, A., & Genest, J. (2017). ATP binding cassette A1 (ABCA1) mediates microparticle formation during high-density lipoprotein (HDL) biogenesis. Atherosclerosis, 257, 90-99.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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