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Mouse Anti-ABCA1 Recombinant Antibody (AB.H10) (CBMAB-0002CQ)

This product is a mouse antibody that recognizes ABCA1. The antibody AB.H10 can be used for immunoassay techniques such as: ELISA, FC, IF, IHC-P, WB.
Host Animal
Mouse
Application
ELISA, FC, IF, IHC-P, WB
Application Notes
The AB.H10 antibody has been reported tested in various applications including WB, IP, IF, IHC, FCM.

For western blot, the recommended concentration is 1:200-1:500. The theoretical molecular weight is 254KDa. The recommended blocking agent is 4% BSA and the recommended total protein is 80-100µg.

For IF and IHC, the recommended dilution is 1:200. Before IHC staining, a kindly reminder that please conduct heat mediated antigen retrieval before commencing.

For flow cytometry, the recommended reported PBS buffer contains 5% FBS, and incubation time is 30 min at 4°C. The recommended concentration is 1-2 µg for 10^6 cells.

For ELISA, the concentration is dependent on the specific assay.

It's recommended that the optimal antibody concentration, dilution, incubation time etc. are best to be carefully titrated and determined by the end user.
Immunogen
Human ABCA1 aa 1800-2260
Antibody Isotype
IgG1
Specificity
Human
Cross Reactivity
Mouse, Rat, Chicken, Bovine, Human
Clone
AB.H10
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Introduction
ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White).The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. cAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport. Mutations in this gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency.
Alternative Name
ATP-Binding Cassette, Sub-Family A (ABC1), Member 1; Cholesterol Efflux Regulatory Protein; ABC-1; ABC1; CERP; ATP-Binding Cassette Transporter A1; ATP-Binding Cassette Transporter 1; ATP-Binding Cassette 1; Tangier Disease; Membrane-Bound; EC 3.6.3.41; EC 2.8.1.8; EC 2.7.7.8; EC 3.6.3; HDLDT1; TGD
Entrez Gene ID
UniProt ID
Cat CBMAB-0002CQ
Conjugates
Size
Price $
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