This HJ1 antibody has been reported in applications of WB, IHC, FACS.
For WB, The recommended RIPA buffer dosage is 10ul/mg,the recommended total protein volumn is 10 ug for higher abundance proteins, and 50-80 ug for lower abundance proteins. The band is seen around 250 kDa. The observed molecular weight may vary due to modifiations etc. The recommended dilution is 1:2000.
For IHC, the Ab has been tested in paraffinembedded lung tissues, and the recommended dilution is 1:400, and incubation time is around 30 mins.
For FCM, the Ab has been tested for human PBMC. The recommended concentration is 1 µg/10^6 cells.
It's recommended that the optimal antibody concentration, dilution, incubation time etc. are best to be carefully titrated in specific assays.
N-terminal extracellular loop of ABCA1
Mouse, Rat, Human
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White).The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. cAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport. Mutations in this gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency.
ATP-Binding Cassette, Sub-Family A (ABC1), Member 1; Cholesterol Efflux Regulatory Protein; ABC-1; ABC1; CERP; ATP-Binding Cassette Transporter A1; ATP-Binding Cassette Transporter 1; ATP-Binding Cassette 1; Tangier Disease; Membrane-Bound; EC 188.8.131.52; EC 184.108.40.206; EC 220.127.116.11; EC 3.6.3; HDLDT1; TGD