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Mouse Anti-ABCA1 Recombinant Antibody (V2-6056) (CBMAB-0001CQ)

This product is a mouse antibody that recognizes ABCA1. The antibody HJ1 can be used for immunoassay techniques such as: WB, FC, IHC-P.
See all ABCA1 antibodies
Published Data
Fig.1 Alterations in CTFβ and Aβ by miR-106b were rescued by overexpression of ABCA1 ORF. (A) and (C) shows the western blot results of ABCA1 using a monoclonal antibody (Cat# CBMAB-0001CQ).
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Fig.2 Loss of protein expression of ABCA1 in PAH PEC and decreased mRNA expression of ABCA1 in lungs from PAH patients. Immunohistochemistry with mouse monoclonal anti-ABCA1 antibody (Cat# CBMAB-0001CQ, Clone HJ1) in healthy or PAH lungs are shown in (A) and (C).
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Fig.1 Immunofluorescence detection of ABCA1 expression in the MDA-MB-231 cell line after 24 h incubation with 30 µM of oxysterols.
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Fig.2 Representative images of ABCA1 immunoreactivity on the surface of WT and PMP22 KO mouse Schwann cells.
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Fig.3 Immunoblot detection of ABCA1 protein expression aftertreatments with vehicle(DMSO), 8-Br-cAMP(cAMP), or CsA.
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Summary

Host Animal
Mouse
Specificity
Mouse, Rat, Human
Clone
V2-6056
Antibody Isotype
IgG2b, κ
Application
WB, FC, IHC-P

Basic Information

Immunogen
N-terminal extracellular loop of ABCA1
Specificity
Mouse, Rat, Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
This HJ1 antibody has been reported in applications of WB, IHC, FACS.
For WB, The recommended RIPA buffer dosage is 10ul/mg,the recommended total protein volumn is 10 ug for higher abundance proteins, and 50-80 ug for lower abundance proteins. The band is seen around 250 kDa. The observed molecular weight may vary due to modifiations etc. The recommended dilution is 1:2000.
For IHC, the Ab has been tested in paraffinembedded lung tissues, and the recommended dilution is 1:400, and incubation time is around 30 mins.
For FCM, the Ab has been tested for human PBMC. The recommended concentration is 1 µg/10^6 cells.
It's recommended that the optimal antibody concentration, dilution, incubation time etc. are best to be carefully titrated in specific assays.
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:2,000
IHC1:400
IHC-P1:400
FC1 µg/10^6 cells

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS
Preservative
0.05% sodium azide
Concentration
1 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ATP Binding Cassette Subfamily A Member 1
Introduction
ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White).The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. cAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport. Mutations in this gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency.
Entrez Gene ID
Human19
Mouse11303
Rat313210
UniProt ID
HumanO95477
MouseP41233
RatF1LNL3
Alternative Names
ATP-Binding Cassette, Sub-Family A (ABC1), Member 1; Cholesterol Efflux Regulatory Protein; ABC-1; ABC1; CERP; ATP-Binding Cassette Transporter A1; ATP-Binding Cassette Transporter 1; ATP-Binding Cassette 1; Tangier Disease; Membrane-Bound; EC 3.6.3.41; EC 2.8.1.8; EC 2.7.7.8; EC 3.6.3; HDLDT1; TGD
Function
Catalyzes the translocation of specific phospholipids from the cytoplasmic to the extracellular/lumenal leaflet of membrane coupled to the hydrolysis of ATP. Thereby, participates in phospholipid transfer to apoliproteins to form nascent high density lipoproteins/HDLs. Transports preferentially phosphatidylcholine over phosphatidylserine. May play a similar role in the efflux of intracellular cholesterol to apoliproteins and the formation of nascent high density lipoproteins/HDLs
Biological Process
Adenylate cyclase-activating G protein-coupled receptor signaling pathway
Cellular response to low-density lipoprotein particle stimulus
Cholesterol efflux
Cholesterol homeostasis
Cholesterol metabolic process
Endosomal transport
Export across plasma membrane
G protein-coupled receptor signaling pathway
High-density lipoprotein particle assembly
Intracellular cholesterol transport
Intracellular receptor signaling pathway
Lipid transport
Lysosome organization
Negative regulation of cholesterol storage
Negative regulation of macrophage derived foam cell differentiation
Phospholipid efflux
Phospholipid homeostasis
Phospholipid translocation
Platelet dense granule organization
Positive regulation of cholesterol efflux
Positive regulation of high-density lipoprotein particle assembly
Protein secretion
Protein transmembrane transport
Regulation of Cdc42 protein signal transduction
Regulation of high-density lipoprotein particle assembly
Regulation of metabolic process
Response to laminar fluid shear stress
Reverse cholesterol transport
Signal release
Cellular Location
Endosome; Cell membrane
Involvement in disease
An autosomal recessive disorder characterized by near absence of plasma high density lipoproteins, low serum HDL cholesterol, and massive tissue deposition of cholesterol esters. Clinical features include large yellow-orange tonsils, hepatomegaly, splenomegaly, enlarged lymph nodes, and often sensory polyneuropathy.
An autosomal dominant disorder characterized by decreased plasma high density lipoproteins, moderately low HDL cholesterol, a reduction in cellular cholesterol efflux, and susceptibility to premature coronary artery disease.
Topology
Helical: 22-42 aa
Extracellular: 43-639 aa
Helical: 640-1371 aa
Extracellular: 1372-1656 aa
Helical: 1657-1872 aa
PTM
Phosphorylation on Ser-2054 regulates phospholipid efflux.
Palmitoylated by ZDHHC8. Palmitoylation is essential for localization to the plasma membrane

Wang, S. M., Lin, W. C., Lin, H. Y., Chen, Y. L., Ko, C. Y., & Wang, J. M. (2021). CCAAT/Enhancer-binding protein delta mediates glioma stem-like cell enrichment and ATP-binding cassette transporter ABCA1 activation for temozolomide resistance in glioblastoma. Cell Death Discovery, 7(1), 1-11.

Li, J., Wen, M., Zhang, Z., Qiu, Z., & Sun, Y. (2020). The R219K polymorphism of the ATP binding cassette subfamily A member 1 gene and susceptibility to ischemic stroke in Chinese population. Open Medicine, 15(1), 274-282.

Jiang, P., Zhao, Z., Li, X., Wang, M., Xia, L., Cao, Y., ... & Fang, X. (2020). RNA interference mediated knockdown of ATP binding cassette subfamily A member 1 decreases the triglyceride content of bovine mammary epithelial cells. Pakistan Journal of Zoology, 52(1), 239.

Chen, Z., Chu, S., Wang, X., Fan, Y., Zhan, T., Arbab, A. A. I., ... & Yang, Z. (2019). MicroRNA-106b regulates milk fat metabolism via ATP binding cassette subfamily A member 1 (ABCA1) in bovine mammary epithelial cells. Journal of agricultural and food chemistry, 67(14), 3981-3990.

Yan, H., Cheng, L., Jia, R., Yao, H., Wu, H., Shen, Y., ... & Zhang, Z. (2019). ATP-binding cassette sub-family a member1 gene mutation improves lipid metabolic abnormalities in diabetes mellitus. Lipids in health and disease, 18(1), 1-6.

Hou, L., Tang, S., Wu, B. J., Ong, K. L., Westerterp, M., Barter, P. J., ... & Rye, K. A. (2019). Apolipoprotein A‐l improves pancreatic β‐cell function independent of the ATP‐binding cassette transporters ABCA1 and ABCG1. The FASEB Journal, 33(7), 8479-8489.

Jung, D., Cao, S., Liu, M., & Park, S. (2018). A meta-analysis of the associations between the ATP-binding cassette transporter ABCA1 R219K (rs2230806) polymorphism and the risk of type 2 diabetes in Asians. Hormone and Metabolic Research, 50(04), 308-316.

Wang, L., Fumoto, T., Masumoto, S., Shoji, T., Miura, T., Naraoka, M., ... & Ohkuma, H. (2017). Regression of atherosclerosis with apple procyanidins by activating the ATP-binding cassette subfamily A member 1 in a rabbit model. Atherosclerosis, 258, 56-64.

Hafiane, A., & Genest, J. (2017). ATP binding cassette A1 (ABCA1) mediates microparticle formation during high-density lipoprotein (HDL) biogenesis. Atherosclerosis, 257, 90-99.

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For research use only. Not intended for any clinical use.

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