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Mouse Anti-ABCA1 Recombinant Antibody (HJ1) (CBMAB-0001CQ)

This product is a mouse antibody that recognizes ABCA1. The antibody HJ1 can be used for immunoassay techniques such as: WB, FC, IHC-P.
Host Animal
Mouse
Application
WB, FC, IHC-P
Application Notes
This HJ1 antibody has been reported in applications of WB, IHC, FACS.

For WB, The recommended RIPA buffer dosage is 10ul/mg,the recommended total protein volumn is 10 ug for higher abundance proteins, and 50-80 ug for lower abundance proteins. The band is seen around 250 kDa. The observed molecular weight may vary due to modifiations etc. The recommended dilution is 1:2000.

For IHC, the Ab has been tested in paraffinembedded lung tissues, and the recommended dilution is 1:400, and incubation time is around 30 mins.

For FCM, the Ab has been tested for human PBMC. The recommended concentration is 1 µg/10^6 cells.

It's recommended that the optimal antibody concentration, dilution, incubation time etc. are best to be carefully titrated in specific assays.
Immunogen
N-terminal extracellular loop of ABCA1
Antibody Isotype
IgG2b, κ
Cross Reactivity
Mouse, Rat, Human
Clone
HJ1
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Introduction
ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White).The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. cAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport. Mutations in this gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency.
Alternative Name
ATP-Binding Cassette, Sub-Family A (ABC1), Member 1; Cholesterol Efflux Regulatory Protein; ABC-1; ABC1; CERP; ATP-Binding Cassette Transporter A1; ATP-Binding Cassette Transporter 1; ATP-Binding Cassette 1; Tangier Disease; Membrane-Bound; EC 3.6.3.41; EC 2.8.1.8; EC 2.7.7.8; EC 3.6.3; HDLDT1; TGD
Entrez Gene ID
UniProt ID
Cat CBMAB-0001CQ
Conjugates
Size
Price $
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