Rabbit Anti-ACAA2 Recombinant Antibody (
12F4) (V2LY-0725-LY1558)

Name: Rabbit Anti-ACAA2 Recombinant Antibody (12F4)
SKU: V2LY-0725-LY1558
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-ACAA2 Recombinant Antibody (12F4) (V2LY-0725-LY1558) in WB

Western Blot Positive WB detected in: Hela whole cell lysate(20µg), A549 whole cell lysate(20µg), HepG2 whole cell lysate(20µg), 293T whole cell lysate(20µg), MCF7 whole cell lysate(20µg), JK whole cell lysate(20µg), Mouse Liver tissue lysate(20µg), Mouse Kidney tissue lysate(20µg) All lanes: ACAA2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 10s

Rabbit Anti-ACAA2 Recombinant Antibody (12F4) (V2LY-0725-LY1558) in IHC

IHC image of V2LY-0725-LY1558 diluted at 1:150 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

Rabbit Anti-ACAA2 Recombinant Antibody (12F4) (V2LY-0725-LY1558) in IHC

IHC image of V2LY-0725-LY1558 diluted at 1:150 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

Rabbit Anti-ACAA2 Recombinant Antibody (12F4) (V2LY-0725-LY1558) in IHC

IHC image of V2LY-0725-LY1558 diluted at 1:150 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

Rabbit Anti-ACAA2 Recombinant Antibody (12F4) (V2LY-0725-LY1558) in IF

Immunofluorescence staining of Hela cell with V2LY-0725-LY1558 at 1:12, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

12F4

Application

ELISA, WB, IHC, IF

Immunogen

A synthesized peptide derived from human ACAA2.

Host Species

Rabbit

Specificity

Human, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC	1:50-1:200
IF	1:10-1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

acetyl-Coenzyme A acyltransferase 2

Entrez Gene ID

10449

UniProt ID

P42765

Function

In the production of energy from fats, this is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA (Probable). Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain unbranched 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms (Probable). Also catalyzes the condensation of two acetyl-CoA molecules into acetoacetyl-CoA and could be involved in the production of ketone bodies (Probable). Also displays hydrolase activity on various fatty acyl-CoAs. Thereby, could be responsible for the production of acetate in a side reaction to beta-oxidation (Probable). Abolishes BNIP3-mediated apoptosis and mitochondrial damage.

Biological Process

Cellular response to hypoxia
Cholesterol biosynthetic process
Fatty acid beta-oxidation
Negative regulation of mitochondrial membrane permeability involved in apoptotic process
Negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway

Cellular Location

Mitochondrion

Symeou, S., Tzamaloukas, O., Banos, G., & Miltiadou, D. (2020). ACAA2 and FASN polymorphisms affect the fatty acid profile of Chios sheep milk. Journal of Dairy Research, 87(1), 23-26.

Choi, S., Pfleger, J., Jeon, Y. H., Yang, Z., He, M., Shin, H., ... & Abdellatif, M. (2019). Oxoglutarate dehydrogenase and acetyl-CoA acyltransferase 2 selectively associate with H2A. Z-occupied promoters and are required for histone modifications. Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms, 1862(10), 194436.

Zhang, Y., Wang, Y., Wang, X., Ji, Y., Cheng, S., Wang, M., ... & Li, B. (2019). Acetyl‐coenzyme A acyltransferase 2 promote the differentiation of sheep precursor adipocytes into adipocytes. Journal of cellular biochemistry, 120(5), 8021-8031.

Fu, S., Chen, Y., Wang, J., Yan, F., Sun, G., Li, Z., ... & Li, G. (2018). Tissue expression profile of miR-124a-3p and its interaction relationship with ACAA2 gene in chicken (Gallus gallus). Journal of Agricultural Biotechnology, 26(6), 949-958.

Miltiadou, D., Hager-Theodorides, A. L., Symeou, S., Constantinou, C., Psifidi, A., Banos, G., & Tzamaloukas, O. (2017). Variants in the 3′ untranslated region of the ovine acetyl-coenzyme A acyltransferase 2 gene are associated with dairy traits and exhibit differential allelic expression. Journal of dairy science, 100(8), 6285-6297.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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