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Rabbit Anti-ACACA (Phosphorylated S80) Recombinant Antibody (V2-556135) (PTM-CBMAB-0640LY)

This antibody is a recombiant antibody against ACACA. The antibody can be used for immunoassay techniques, such as WB, IP, IHC-p, IF.
See all ACACA antibodies
Published Data
Rabbit Anti-ACACA (Phosphorylated S80) Recombinant Antibody (D7D11) (PTM-CBMAB-0640LY) in WB
Representative western blot showing the increased Nox2, p-AMPK, and p-ACC1 level in 6-PGDH-depleted AGS cells. ...View More
Chen, C., Du, P., Zhang, Z., & Bao, D. (2023). 6-Phosphogluconate dehydrogenase inhibition arrests growth and induces apoptosis in gastric cancer via AMPK activation and oxidative stress. Open Life Sciences, 18(1), 20220514.
Rabbit Anti-ACACA (Phosphorylated S80) Recombinant Antibody (D7D11) (PTM-CBMAB-0640LY) in IHC
Representative staining of several metabolic enzymes (ACC-acetyl-CoA carboxylase showed high tissue heterogeneity in the studied breast cancer cases. 3,30 -diaminobenzidine (DAB) (brown) was used as a chromogen. Scale bars are given in the figures. ...View More
Dankó, T., Petővári, G., Raffay, R., Sztankovics, D., Moldvai, D., Vetlényi, E., ... & Sebestyén, A. (2022). Characterisation of 3D bioprinted human breast cancer model for in vitro drug and metabolic targeting. International Journal of Molecular Sciences, 23(13), 7444.

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
V2-556135
Antibody Isotype
IgG
Application
WB, IP, IHC-p, IF

Basic Information

Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Ser79 of human acetyl-CoA carboxylase protein.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IP1:500-1:5,000
IHC-P1:200-1:800
IF(ICC)1:100-1:400

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Acetyl-CoA Carboxylase Alpha
Introduction
Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human31
Mouse107476
Rat60581
UniProt ID
HumanQ13085
MouseQ5SWU9
RatP11497
Function
Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis. This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA.
Biological Process
Acetyl-CoA metabolic process
Carnitine shuttle
Cellular response to prostaglandin E stimulus
Fatty acid biosynthetic process
Fatty-acyl-CoA biosynthetic process
Lipid homeostasis
Malonyl-CoA biosynthetic process
Positive regulation of cellular metabolic process
Protein homotetramerization
Protein metabolic process
Regulation of lipid metabolic process
Tissue homeostasis
Cellular Location
Cytosol
Involvement in disease
An inborn error of de novo fatty acid synthesis associated with severe brain damage, persistent myopathy and poor growth.
PTM
Phosphorylation on Ser-1263 is required for interaction with BRCA1.
Phosphorylation at Ser-80 by AMPK inactivates enzyme activity.
The biotin cofactor is covalently attached to the central biotinyl-binding domain and is required for the catalytic activity.

Prasad, A. R., Bhattacharya, T., Chatterjee, R. N., Divya, D., Bhanja, S. K., Shanmugam, M., & Sagar, N. G. (2021). Silencing Acetyl-CoA carboxylase A (ACACA) and Sterol Regulatory Element-binding Protein 1 (SREBP1) Genes Through RNAi Reduce Cholesterol Content in Serum and Eggs of Transgenic Chicken.

Azis, R., Anggraeni, A., & Gunawan, A. (2020). Acetyl-CoA Carboxylase Alpha Gene Polymorphism and Its Association with Milk Fatty Acid of Holstein Friesian Using Real-Time PCR Method. Tropical Animal Science Journal, 43(4), 306-313.

Cantley, J., Davenport, A., Vetterli, L., Nemes, N. J., Whitworth, P. T., Boslem, E., ... & Biden, T. J. (2019). Disruption of beta cell acetyl-CoA carboxylase-1 in mice impairs insulin secretion and beta cell mass. Diabetologia, 62(1), 99-111.

Prasad, A. R., Bhattacharya, T. K., Kumar, P., Sagar, N. G., Bhushan, B., Vishnu, P. G., & Devara, D. (2018). Expression Profile of Acetyl-CoA Carboxylase A (ACACA) Gene in Layer Chicken during Juvenile Stage. Journal of Animal Research, 8(3), 525-529.

Raimondo, S., Saieva, L., Cristaldi, M., Monteleone, F., Fontana, S., & Alessandro, R. (2018). Label-free quantitative proteomic profiling of colon cancer cells identifies acetyl-CoA carboxylase alpha as antitumor target of Citrus limon-derived nanovesicles. Journal of proteomics, 173, 1-11.

Kęsek, M. M., Smołucha, G., & Zielak-Steciwko, A. E. (2017). Acetyl-CoA Carboxylase α and Stearoyl-CoA Desaturase genes polymorphism and their influence on fatty acid profile in milk of Polish Holstein-Friesian cows. Annals of Animal Science, 17(4), 993.

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For research use only. Not intended for any clinical use.

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