Human ACACA ELISA Kit (V2LY-0626-LY3661)

Name: Human ACACA ELISA Kit
SKU: V2LY-0626-LY3661
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0023 ng/mL

Detection Range

0.005-1 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

265.6 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Acetyl-CoA Carboxylase Alpha

Function

Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis. This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA.

Biological Process

Acetyl-CoA metabolic process
Carnitine shuttle
Cellular response to prostaglandin E stimulus
Fatty acid biosynthetic process
Fatty-acyl-CoA biosynthetic process
Lipid homeostasis
Malonyl-CoA biosynthetic process
Positive regulation of cellular metabolic process
Protein homotetramerization
Protein metabolic process
Regulation of lipid metabolic process
Tissue homeostasis

Cellular Location

Cytosol

Involvement in disease

An inborn error of de novo fatty acid synthesis associated with severe brain damage, persistent myopathy and poor growth.

PTM

Phosphorylation on Ser-1263 is required for interaction with BRCA1.
Phosphorylation at Ser-80 by AMPK inactivates enzyme activity.
The biotin cofactor is covalently attached to the central biotinyl-binding domain and is required for the catalytic activity.

Prasad, A. R., Bhattacharya, T., Chatterjee, R. N., Divya, D., Bhanja, S. K., Shanmugam, M., & Sagar, N. G. (2021). Silencing Acetyl-CoA carboxylase A (ACACA) and Sterol Regulatory Element-binding Protein 1 (SREBP1) Genes Through RNAi Reduce Cholesterol Content in Serum and Eggs of Transgenic Chicken.

Azis, R., Anggraeni, A., & Gunawan, A. (2020). Acetyl-CoA Carboxylase Alpha Gene Polymorphism and Its Association with Milk Fatty Acid of Holstein Friesian Using Real-Time PCR Method. Tropical Animal Science Journal, 43(4), 306-313.

Cantley, J., Davenport, A., Vetterli, L., Nemes, N. J., Whitworth, P. T., Boslem, E., ... & Biden, T. J. (2019). Disruption of beta cell acetyl-CoA carboxylase-1 in mice impairs insulin secretion and beta cell mass. Diabetologia, 62(1), 99-111.

Prasad, A. R., Bhattacharya, T. K., Kumar, P., Sagar, N. G., Bhushan, B., Vishnu, P. G., & Devara, D. (2018). Expression Profile of Acetyl-CoA Carboxylase A (ACACA) Gene in Layer Chicken during Juvenile Stage. Journal of Animal Research, 8(3), 525-529.

Raimondo, S., Saieva, L., Cristaldi, M., Monteleone, F., Fontana, S., & Alessandro, R. (2018). Label-free quantitative proteomic profiling of colon cancer cells identifies acetyl-CoA carboxylase alpha as antitumor target of Citrus limon-derived nanovesicles. Journal of proteomics, 173, 1-11.

Kęsek, M. M., Smołucha, G., & Zielak-Steciwko, A. E. (2017). Acetyl-CoA Carboxylase α and Stearoyl-CoA Desaturase genes polymorphism and their influence on fatty acid profile in milk of Polish Holstein-Friesian cows. Annals of Animal Science, 17(4), 993.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Rat Acaca ELISA Kit (CAT#: V2LY-0626-LY1301)

Mouse Acaca ELISA Kit (CAT#: V2LY-0626-LY1505)

Rat Acaca ELISA Kit (2) (CAT#: V2LY-0626-LY2027)

Chicken ACACA ELISA Kit (CAT#: V2LY-0626-LY312)

Related Products

Mouse Anti-ACACA Recombinant Antibody (V2-179158) (CAT#: CBMAB-A0413-YC)

Mouse Anti-ACACA Recombinant Antibody (V2-179165) (CAT#: CBMAB-A0420-YC)

Rabbit Anti-ACACA Recombinant Antibody (V2-179243) (CAT#: CBMAB-A0513-YC)

Rabbit Anti-ACACA Recombinant Antibody (V2-179161) (CAT#: CBMAB-A0416-YC)

Mouse Anti-ACACA Recombinant Antibody (V2-179242) (CAT#: CBMAB-A0512-YC)

Rabbit Anti-ACACA (Phosphorylated S80) Recombinant Antibody (V2-556135) (CAT#: PTM-CBMAB-0640LY)

Mouse Acaca ELISA Kit (CAT#: V2LY-0626-LY1505)

Rat Acaca ELISA Kit (CAT#: V2LY-0626-LY1301)

Chicken ACACA ELISA Kit (CAT#: V2LY-0626-LY312)

Rabbit Anti-ACACA Recombinant Antibody (V2-630605) (CAT#: CBMAB-BR002LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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