What buffer should be used for diluting the Mouse Anti-ACAT2 Recombinant Antibody (V2-179219)?

For dilution, it's generally recommended to use a standard antibody dilution buffer, such as PBS (Phosphate-Buffered Saline) containing 0.05% Tween-20 and 1-5% BSA (Bovine Serum Albumin) or non-fat milk as a blocking agent, depending on your specific application (e.g., milk for Western Blot, BSA for immunofluorescence). Always check the product datasheet for the manufacturer's specific recommendations for the best performance.

Is this antibody validated for use in frozen tissue sections or only paraffin-embedded samples?

The Mouse Anti-ACAT2 Recombinant Antibody (V2-179219) has generally been validated for applications like Western Blot and Immunocytochemistry. For immunohistochemistry (IHC), its performance can vary depending on tissue fixation methods (e.g., formalin-fixed paraffin-embedded (FFPE) vs. frozen sections). While many antibodies work across different preparations, it's crucial to consult the product's official datasheet on the Creative Biolabs website, as it will explicitly state the validated tissue types and any necessary antigen retrieval protocols for IHC applications. If your specific tissue type or fixation method isn't listed, it might require empirical optimization or a trial sample.

Mouse Anti-ACAT2 Recombinant Antibody (V2-179219) (CBMAB-A0480-YC)

See all ACAT2 antibodies

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-179219

Application

FC, IF, IHC, WB

Immunogen

Full length recombinant protein of human ACAT2 (NP_0058826) produced in HEK293T cell.

Specificity

Human, Dog, Rat, Monkey

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:2,000
IF(ICC)	1:100
IHC-P	1:50
FC	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Buffer

PBS, pH7.3, 1% BSA, 50% Glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Acetyl-CoA Acetyltransferase 2

Introduction

ACAT2 is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of

Entrez Gene ID

Human	39
Dog	484063
Rat	308100
Monkey	741276

UniProt ID

Human	O75908
Dog	F1Q466
Rat	Q08755
Monkey	O77759

Alternative Names

Acetyl-CoA Acetyltransferase 2; EC 2.3.1.9; Acetyl-Coenzyme A Acetyltransferase 2 (Acetoacetyl Coenzyme A Thiolase); Acetyl-Coenzyme A Acetyltransferase 2; Acetyl-CoA Transferase-Like Protein; Cytosolic Acetoacetyl-CoA Thiolase; Acetoacetyl Coenzyme A Thi

Function

Involved in the biosynthetic pathway of cholesterol.

Biological Process

Cholesterol biosynthetic process
Fatty acid beta-oxidation
Lipid metabolic process

Cellular Location

Cytosol

Zhang, Y., Wei, W., Fan, J., Jin, C., Lu, L., & Fang, W. (2020). Aspergillus fumigatus mitochondrial acetyl coenzyme A acetyltransferase as an antifungal target. Applied and environmental microbiology, 86(7), e02986-19.

Ibrahim, A., Shafie, N. H., Mohd Esa, N., Shafie, S. R., Bahari, H., & Abdullah, M. A. (2020). Mikania micrantha Extract Inhibits HMG-CoA Reductase and ACAT2 and Ameliorates Hypercholesterolemia and Lipid Peroxidation in High Cholesterol-Fed Rats. Nutrients, 12(10), 3077.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

ACAT2 Matched Antibody Pair (002) (CAT#: APMAB-002LY)

ACAT2 Matched Antibody Pair (003) (CAT#: APMAB-003LY)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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