Mouse Anti-ACAT2 Recombinant Antibody (V2-179228) (CBMAB-A0489-YC)

Name: Mouse Anti-ACAT2 Recombinant Antibody (V2-179228)
SKU: CBMAB-A0489-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-179228

Application

WB, ELISA

Immunogen

Recombinant human ACAT2 (1-397 aa) purified from E. coli

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000
IF(ICC)	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH7.4, 10% Glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Acetyl-CoA Acetyltransferase 2

Introduction

ACAT2 is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of

Entrez Gene ID

UniProt ID

Q9BWD1

Alternative Names

Acetyl-CoA Acetyltransferase 2; EC 2.3.1.9; Acetyl-Coenzyme A Acetyltransferase 2 (Acetoacetyl Coenzyme A Thiolase); Acetyl-Coenzyme A Acetyltransferase 2; Acetyl-CoA Transferase-Like Protein; Cytosolic Acetoacetyl-CoA Thiolase; Acetoacetyl Coenzyme A Thi

Function

Involved in the biosynthetic pathway of cholesterol.

Biological Process

Cholesterol biosynthetic process
Fatty acid beta-oxidation
Lipid metabolic process

Cellular Location

Cytosol

Zhang, Y., Wei, W., Fan, J., Jin, C., Lu, L., & Fang, W. (2020). Aspergillus fumigatus mitochondrial acetyl coenzyme A acetyltransferase as an antifungal target. Applied and environmental microbiology, 86(7), e02986-19.

Ibrahim, A., Shafie, N. H., Mohd Esa, N., Shafie, S. R., Bahari, H., & Abdullah, M. A. (2020). Mikania micrantha Extract Inhibits HMG-CoA Reductase and ACAT2 and Ameliorates Hypercholesterolemia and Lipid Peroxidation in High Cholesterol-Fed Rats. Nutrients, 12(10), 3077.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

ACAT2 Matched Antibody Pair (002) (CAT#: APMAB-002LY)

ACAT2 Matched Antibody Pair (003) (CAT#: APMAB-003LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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