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Mouse Anti-ACOT13 Recombinant Antibody (V2-347934) (CBMAB-T0005-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to ACOT13 (Acyl-CoA Thioesterase 13). The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all ACOT13 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Rat, Mouse
Clone
V2-347934
Antibody Isotype
IgG1
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Specific for an epitope mapping between amino acids 118-140 at the C-terminus of THEM2 of human origin
Specificity
Human, Rat, Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 118-140

Target

Full Name
Acyl-CoA Thioesterase 13
Introduction
ACOT13 is a member of the thioesterase superfamily. In humans, ACOT13 co-localizes with microtubules and is essential for sustained cell proliferation. The orthologous mouse protein forms a homotetramer and is associated with mitochondria. The mouse protein plays a role as a medium- and long-chain acyl-CoA thioesterase.
Entrez Gene ID
Human55856
Mouse66834
Rat291135
UniProt ID
HumanQ9NPJ3
MouseQ9CQR4
Rat
Alternative Names
Acyl-CoA Thioesterase 13; Thioesterase Superfamily Member 2; THEM2; Acyl-Coenzyme A Thioesterase 13; Hypothalamus Protein HT012; EC 3.1.2.-; PNAS-27; HT012
Function
Catalyzes the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates (By similarity). Can also hydrolyze 3-hydroxyphenylacetyl-CoA and 3,4-dihydroxyphenylacetyl-CoA (in vitro) (By similarity). May play a role in controlling adaptive thermogenesis (By similarity).
Biological Process
Acyl-CoA metabolic process
Negative regulation of cold-induced thermogenesis
Protein homotetramerization
Cellular Location
Nucleus; Mitochondrion; Spindle; Cytosol. During interphase, found both in the nucleus and in the cytoplasm. At mitosis, localizes to the spindle. Colocalizes with tubulin.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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