Human Recombinant ACOT13 protein, His Tag (V2LY-0526-LY1902)

Name: Human Recombinant ACOT13 protein, His Tag
SKU: V2LY-0526-LY1902
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant ACOT13 protein, His Tag consist of Amino Acid: 1-140 and predicts a molecular mass of 17.2 kDa.

Molecule Mass

17.2 kDa

Sequence

Amino Acid: 1-140

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Acyl-CoA Thioesterase 13

Function

Catalyzes the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates (By similarity). Can also hydrolyze 3-hydroxyphenylacetyl-CoA and 3,4-dihydroxyphenylacetyl-CoA (in vitro) (By similarity). May play a role in controlling adaptive thermogenesis (By similarity).

Biological Process

Acyl-CoA metabolic process
Negative regulation of cold-induced thermogenesis
Protein homotetramerization

Cellular Location

Nucleus; Mitochondrion; Spindle; Cytosol. During interphase, found both in the nucleus and in the cytoplasm. At mitosis, localizes to the spindle. Colocalizes with tubulin.

Bekeova, C., Anderson-Pullinger, L., Boye, K., Boos, F., Sharpadskaya, Y., Herrmann, J. M., & Seifert, E. L. (2019). Multiple acyl-CoA thioesterases occupy distinct functional niches within the mitochondrial matrix. bioRxiv, 705327.

Sun, G., Li, F., Ma, X., Sun, J., Jiang, R., Tian, Y., ... & Li, W. (2019). gga-miRNA-18b-3p inhibits intramuscular adipocytes differentiation in chicken by targeting the ACOT13 gene. Cells, 8(6), 556.

Hung, J. Y., Chiang, S. R., Liu, K. T., Tsai, M. J., Huang, M. S., Shieh, J. M., ... & Hsu, Y. L. (2017). Overexpression and proliferation dependence of acyl‑CoA thioesterase 11 and 13 in lung adenocarcinoma. Oncology letters, 14(3), 3647-3656.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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