Mouse Anti-ACOT7 Recombinant Antibody (V2-179347) (CBMAB-A0648-YC)

Provided herein is a Mouse monoclonal antibody against Human Acyl-CoA Thioesterase 7. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all ACOT7 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

V2-179347

Antibody Isotype

IgG2a

Application

ELISA, WB

Basic Information

Immunogen

Recombinant human ACOT7 (1-370aa) purified from E. coli

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:500-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH7.4, 10% glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Acyl-CoA Thioesterase 7

Introduction

ACOT7 is a member of the acyl coenzyme family. The encoded protein hydrolyzes the CoA thioester of palmitoyl-CoA and other long-chain fatty acids. Decreased expression of this gene may be associated with mesial temporal lobe epilepsy. Alternatively splice

Entrez Gene ID

11332

UniProt ID

O00154

Alternative Names

Acyl-CoA Thioesterase 7; Long Chain Acyl-CoA Thioester Hydrolase; Brain Acyl-CoA Hydrolase; EC 3.1.2.2; CTE-IIa; CTE-II; BACH; Cytosolic Acyl Coenzyme A Thioester Hydrolase; Acyl-CoA Thioesterase, Long Chain;

Function

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Acyl-coenzyme A thioesterase 7/ACOT7 preferentially hydrolyzes palmitoyl-CoA, but has a broad specificity acting on other fatty acyl-CoAs with chain-lengths of C8-C18. May play an important physiological function in brain.

Biological Process

Acyl-CoA metabolic process
Coenzyme A biosynthetic process
Fatty acid metabolic process
Long-chain fatty-acyl-CoA catabolic process
Medium-chain fatty acid biosynthetic process
Medium-chain fatty-acyl-CoA catabolic process
Palmitic acid biosynthetic process

Cellular Location

Isoform 1: Mitochondrion
Isoform 4: Cytosol
Isoform 5: Mitochondrion
Isoform 6: Cytosol

References

Feng, H., & Liu, X. (2020). Interaction between ACOT7 and LncRNA NMRAL2P via methylation regulates gastric cancer progression. Yonsei Medical Journal, 61(6), 471.

Bowman, C. E., Selen Alpergin, E. S., Ellis, J. M., & Wolfgang, M. J. (2019). Loss of ACOT7 potentiates seizures and metabolic dysfunction. American Journal of Physiology-Endocrinology and Metabolism, 317(5), E941-E951.

Bakshi, I., Brown, S. H., Brandon, A. E., Suryana, E., Mitchell, T. W., Turner, N., & Cooney, G. J. (2018). Increasing Acyl CoA thioesterase activity alters phospholipid profile without effect on insulin action in skeletal muscle of rats. Scientific reports, 8(1), 1-12.

Jung, S. H., Lee, H. C., Hwang, H. J., Park, H. A., Moon, Y. A., Kim, B. C., ... & Lee, J. S. (2017). Acyl-CoA thioesterase 7 is involved in cell cycle progression via regulation of PKC ζ–p53–p21 signaling pathway. Cell death & disease, 8(5), e2793-e2793.

Wall, V. Z., Barnhart, S., Kramer, F., Kanter, J. E., Vivekanandan-Giri, A., Pennathur, S., ... & Bornfeldt, K. E. (2017). Inflammatory stimuli induce acyl-CoA thioesterase 7 and remodeling of phospholipids containing unsaturated long (≥ C20)-acyl chains in macrophages [S]. Journal of lipid research, 58(6), 1174-1185.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

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Mouse Anti-ACOT7 Recombinant Antibody (V2-179346)

Mouse Anti-ACOT7 Recombinant Antibody (V2-179345)

Mouse Anti-ACOT7 Recombinant Antibody (V2-179344)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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