Has this antibody been validated in applications other than Western Blot (WB) and Immunoprecipitation (IP)?

Currently, this antibody has been validated for use in WB and IP. We have not yet tested it in other applications. If you are interested in using this antibody in an unlisted application, we would recommend testing it at a small scale first.

Rabbit Anti-ACSL1 Recombinant Antibody (V2-179443) (CBMAB-A0750-YC)

Q: What is the immunogen used to generate this antibody?

This antibody was generated using a synthetic peptide corresponding to residues surrounding Ala257 of the human ACSL1 protein.

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Published Data

Rabbit Anti-ACSL1 Recombinant Antibody (D2H5) (CBMAB-A0750-YC) in WB

Immunoblot analysis of acyl-CoA ligase (ACSL) . ...View More

Lizardo, K., Ayyappan, J. P., Oswal, N., Weiss, L. M., Scherer, P. E., & Nagajyothi, J. F. (2021). Fat tissue regulates the pathogenesis and severity of cardiomyopathy in murine chagas disease. PLoS neglected tropical diseases, 15(4), e0008964.

Datasheet

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Basic Information

Host Animal

Rabbit

Clone

V2-179443

Application

WB, IP

Immunogen

Synthetic peptide corresponding to residues surrounding Ala257 of human ACSL1 protein.

Specificity

Human, Mouse, Rat, Monkey

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000
IP	1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

acyl-CoA synthetase long-chain family member 1

Introduction

ACSL1 is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA

Entrez Gene ID

Human	2180
Mouse	14081
Rat	25288
Monkey	694871

UniProt ID

Human	P33121
Mouse	P41216
Rat	P18163
Monkey	H2QQI4

Alternative Names

Acyl-CoA Synthetase Long Chain Family Member 1; Fatty-Acid-Coenzyme A Ligase, Long-Chain 2; Long-Chain Fatty-Acid-Coenzyme A Ligase 1; Long-Chain Fatty Acid-CoA Ligase 2; Long-Chain Acyl-CoA Synthetase 1; Long-Chain Acyl-CoA Synthetase 2; Lignoceroyl-CoA

Function

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate. Preferentially activates arachidonate than epoxyeicosatrienoic acids (EETs) or hydroxyeicosatrienoic acids (HETEs) (By similarity).

Biological Process

Adiponectin-activated signaling pathway
Alpha-linolenic acid metabolic process
Fatty acid transport
Linoleic acid metabolic process
Lipid biosynthetic process
Long-chain fatty acid import into cell
Long-chain fatty acid metabolic process
Long-chain fatty-acyl-CoA biosynthetic process
Positive regulation of cold-induced thermogenesis
Positive regulation of long-chain fatty acid import across plasma membrane
Positive regulation of protein serine/threonine kinase activity
Regulation of metabolic process
Response to drug
Response to nutrient
Response to oleic acid
Response to organic cyclic compound
Triglyceride biosynthetic process
Very long-chain fatty acid metabolic process
Xenobiotic catabolic process

Cellular Location

Peroxisome membrane; Microsome membrane; Endoplasmic reticulum membrane; Mitochondrion outer membrane

Topology

Helical: 25-45 aa
Cytoplasmic: 46-698 aa

Dykstra, H., Fisk, C., LaRose, C., Waldhart, A., Meng, X., Zhao, G., & Wu, N. (2021). Mouse long-chain acyl-CoA synthetase 1 is active as a monomer. Archives of Biochemistry and Biophysics, 700, 108773.

Li, B., Liu, J., Xin, X., Zhang, L., Zhou, J., Xia, C., ... & Yu, H. (2021). MiR-34c promotes hepatic stellate cell activation and Liver Fibrogenesis by suppressing ACSL1 expression. International Journal of Medical Sciences, 18(3), 615.

Huh, J. Y., Reilly, S. M., Abu-Odeh, M., Murphy, A. N., Mahata, S. K., Zhang, J., ... & Saltiel, A. R. (2020). TANK-Binding Kinase 1 Regulates the Localization of Acyl-CoA Synthetase ACSL1 to Control Hepatic Fatty Acid Oxidation. Cell Metabolism, 32(6), 1012-1027.

Guo, L., Lu, J., Gao, J., Li, M., Wang, H., & Zhan, X. (2020). The function of SNHG7/miR‐449a/ACSL1 axis in thyroid cancer. Journal of cellular biochemistry, 121(10), 4034-4042.

Singh, A. B., Dong, B., Xu, Y., Zhang, Y., & Liu, J. (2019). Identification of a novel function of hepatic long-chain acyl-CoA synthetase-1 (ACSL1) in bile acid synthesis and its regulation by bile acid-activated farnesoid X receptor. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 1864(3), 358-371.

Roelands, J., Garand, M., Hinchcliff, E., Ma, Y., Shah, P., Toufiq, M., ... & Chaussabel, D. (2019). Long-chain acyl-CoA synthetase 1 role in sepsis and immunity: perspectives from a parallel review of public transcriptome datasets and of the literature. Frontiers in immunology, 10, 2410.

Yang, G., Wang, Y., Feng, J., Liu, Y., Wang, T., Zhao, M., ... & Zhang, X. (2017). Aspirin suppresses the abnormal lipid metabolism in liver cancer cells via disrupting an NFκB-ACSL1 signaling. Biochemical and biophysical research communications, 486(3), 827-832.

Wang, Y., Cai, X., Zhang, S., Cui, M., Liu, F., Sun, B., ... & Ye, L. (2017). HBXIP up-regulates ACSL1 through activating transcriptional factor Sp1 in breast cancer. Biochemical and biophysical research communications, 484(3), 565-571.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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