What are the recommended dilutions for this antibody in Western Blot (WB) and Immunohistochemistry (IHC-P)?

For Western Blotting, we recommend a starting dilution of 1:500-1:3,000. For Immunohistochemistry on paraffin-embedded sections (IHC-P), we suggest a dilution of 1:100-1:1,000. However, the optimal dilution should be determined experimentally by the end-user.

What is the immunogen used to generate this antibody?

This antibody was generated against a synthetic peptide corresponding to a sequence within the C-terminus region of human ACSL4. For more specific information on the immunogen sequence, please contact our technical support team.

Mouse Anti-ACSL4 Recombinant Antibody (V2-161661) (CBMAB-F1646-CQ)

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Datasheet

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Basic Information

Host Animal

Mouse

Clone

V2-161661

Application

IHC-P, WB

Immunogen

Synthetic peptide corresponding to a sequence within the C-terminus region of human FACL4

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:500-1:3,000
IHC-P	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Acyl-CoA Synthetase Long Chain Family Member 4

Introduction

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants.

Entrez Gene ID

Human	2182
Mouse	50790
Rat	113976

UniProt ID

Human	O60488
Mouse	Q9QUJ7
Rat	O35547

Alternative Names

Acyl-CoA Synthetase Long Chain Family Member 4; Fatty-Acid-Coenzyme A Ligase, Long-Chain 4; Long-Chain Fatty-Acid-Coenzyme A Ligase 4; Long-Chain Acyl-CoA Synthetase 4; Lignoceroyl-CoA Synthase; EC 6.2.1.3; LACS 4; FACL4; LACS4;

Function

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially activates arachidonate and eicosapentaenoate as substrates. Preferentially activates 8,9-EET> 14,15-EET> 5,6-EET> 11,12-EET. Modulates glucose-stimulated insulin secretion by regulating the levels of unesterified EETs (By similarity). Modulates prostaglandin E2 secretion.

Biological Process

Dendritic spine development
Embryonic process involved in female pregnancy
Fatty acid metabolic process
Fatty acid transport
Lipid biosynthetic process
Lipid metabolic process
Long-chain fatty acid metabolic process
Long-chain fatty-acyl-CoA biosynthetic process
Long-chain fatty-acyl-CoA metabolic process
Negative regulation of prostaglandin secretion
Neuron differentiation
Positive regulation of cell growth
Positive regulation of insulin secretion
Response to interleukin-15
Response to nutrient
Triglyceride biosynthetic process

Cellular Location

Cell membrane; Microsome membrane; Endoplasmic reticulum membrane; Peroxisome membrane; Mitochondrion outer membrane

Involvement in disease

Mental retardation, X-linked 63 (MRX63): A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. Intellectual deficiency is the only primary symptom of non-syndromic X-linked mental retardation, while syndromic mental retardation presents with associated physical, neurological and/or psychiatric manifestations.
Alport syndrome with mental retardation, midface hypoplasia and elliptocytosis (ATS-MR): An X-linked contiguous gene deletion syndrome characterized by glomerulonephritis, sensorineural hearing loss, mental retardation, midface hypoplasia and elliptocytosis.

Topology

Helical: 8-28 aa
Cytoplasmic: 29-711 aa

Qu, X. F., Liang, T. Y., Wu, D. G., Lai, N. S., Deng, R. M., Ma, C., ... & Chen, G. (2021). Acyl‐CoA synthetase long chain family member 4 plays detrimental role in early brain injury after subarachnoid hemorrhage in rats by inducing ferroptosis. CNS Neuroscience & Therapeutics, 27(4), 449-463.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-ACSL4 Recombinant Antibody (V2-161662) (CAT#: CBMAB-F1647-CQ)

Rabbit Anti-ACSL4 Recombinant Antibody (V2-160818) (CAT#: CBMAB-F0745-CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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