What is the recommended working concentration for this antibody in different applications?

Based on standard protocols and our own validation, we recommend the following dilution ranges as a starting point: Western Blot (WB): 1:5000 to 1:10000 Immunohistochemistry (IHC): 1:200 to 1:500 Immunofluorescence (IF): 1:100 to 1:250 For optimal results, a user-specific titration curve is always recommended to determine the ideal concentration for your unique experimental conditions and sample type.

Is the immunogen sequence for this antibody available?

The immunogen sequence for the Mouse Anti-ACTN3 Recombinant Antibody (V2-630609) is a proprietary, recombinant protein fragment of the human ACTN3 protein. While the full sequence is not publicly disclosed due to proprietary information, this recombinant design ensures high specificity and affinity compared to antibodies raised against short peptides.

Mouse Anti-ACTN3 Recombinant Antibody (V2-630609) (CBMAB-BR008LY)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-630609

Application

IHC, WB

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human ACTN3

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC-P	0.5-0.1 μg/ml
WB	0.1-0.5 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4

Preservative

0.05mg sodium azide

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Actinin Alpha 3 (Gene/Pseudogene)

Introduction

This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status. [provided by RefSeq, Feb 2014]

Entrez Gene ID

Human	89
Mouse	11474
Rat	171009

UniProt ID

Human	Q08043
Mouse	O88990
Rat	Q8R4I6

Alternative Names

Alpha-actinin-3; Alpha-actinin skeletal muscle isoform 3; F-actin cross-linking protein; ACTN3

Function

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

Biological Process

Focal adhesion assembly
Muscle filament sliding
Negative regulation of calcineurin-NFAT signaling cascade
Negative regulation of cold-induced thermogenesis
Negative regulation of glycolytic process
Negative regulation of oxidative phosphorylation
Positive regulation of glucose catabolic process to lactate via pyruvate
Positive regulation of skeletal muscle tissue growth
Regulation of aerobic respiration
Regulation of apoptotic process
Regulation of the force of skeletal muscle contraction
Response to denervation involved in regulation of muscle adaptation
Skeletal muscle atrophy
Transition between fast and slow fiber

Cellular Location

Actin filament; Cytosol; Extracellular exosome; Focal adhesion

Seto, J. T., Roeszler, K. N., Meehan, L. R., Wood, H. D., Tiong, C., Bek, L., ... & North, K. N. (2021). ACTN3 genotype influences skeletal muscle mass regulation and response to dexamethasone. Science Advances, 7(27), eabg0088.

Habibullayevna, B. G., Sobitjanovna, A. S., Makhmusovna, M., & Yusubjanovna, Z. (2020). Optimization Of Molecular-Genetic Methods For The Determination Of Resistance Markers Using Genotyping Of Actn3 And Ace Genes. European Journal of Molecular & Clinical Medicine, 7(10), 67-74.

Shumna, T. Y., Voznyi, O. V., Zinchenko, T. P., & Kamyshnyi, O. M. (2020). Frequency analysis of the ACTN3 gene polymorphism in children with isolated or combined orthodontic pathology with allergic rhinitis. Journal of Education, Health and Sport, 10(7), 41-55.

Massidda, M., Voisin, S., Culigioni, C., Piras, F., Cugia, P., Yan, X., ... & Calò, C. M. (2019). ACTN3 R577X polymorphism is associated with the incidence and severity of injuries in professional football players. Clinical Journal of Sport Medicine, 29(1), 57-61.

Ropka-Molik, K., Stefaniuk-Szmukier, M., Musiał, A. D., Piórkowska, K., & Szmatoła, T. (2019). Sequence analysis and expression profiling of the equine ACTN3 gene during exercise in Arabian horses. Gene, 685, 149-155.

Del Coso, J., Hiam, D., Houweling, P., Pérez, L. M., Eynon, N., & Lucia, A. (2019). More than a ‘speed gene’: ACTN3 R577X genotype, trainability, muscle damage, and the risk for injuries. European journal of applied physiology, 119(1), 49-60.

Ma, T., Lu, D., Zhu, Y. S., Chu, X. F., Wang, Y., Shi, G. P., ... & Wang, X. F. (2018). ACTN3 genotype and physical function and frailty in an elderly Chinese population: the Rugao Longevity and Ageing Study. Age and ageing, 47(3), 416-422.

Eroğlu, O., Zileli, R., Nalbant, M. A., & Ulucan, K. (2018). Prevalence of alpha actinin-3 gene (ACTN3) R577X and angiotensin converting enzyme (ACE) insertion/deletion gene polymorphisms in national and amateur Turkish athletes. Cellular and Molecular Biology, 64(5), 24-28.

Houweling, P. J., Papadimitriou, I. D., Seto, J. T., Pérez, L. M., Coso, J. D., North, K. N., ... & Eynon, N. (2018). Is evolutionary loss our gain? The role of ACTN3 p. Arg577Ter (R577X) genotype in athletic performance, ageing, and disease. Human mutation, 39(12), 1774-1787.

Garton, F. C., Houweling, P. J., Vukcevic, D., Meehan, L. R., Lee, F. X., Lek, M., ... & North, K. N. (2018). The effect of ACTN3 gene doping on skeletal muscle performance. The American Journal of Human Genetics, 102(5), 845-857.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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