Rabbit Anti-ACTN3 Recombinant Antibody (V2-179648) (CBMAB-A0995-YC)

Name: Rabbit Anti-ACTN3 Recombinant Antibody (V2-179648)
SKU: CBMAB-A0995-YC
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Published Data

Rabbit Anti-ACTN3 Recombinant Antibody (CBYC-A261) (CBMAB-A0995-YC) in WB

ACTN3 deficiency is accompanied by a shift toward a slower skeletal muscle phenotype

(A-D) Summary data (mean ± SEM) and representative western blots of the SR Ca2+-handling proteins SERCA1, SERCA2a, CSQ, and CSQ2 in muscle of RR (n = 8) and XX (n = 7) individuals. Band intensities were normalized to their respective myosin loading controls. Data expressed relative to the mean value of the RR group, which was set to 1.0. Statistical difference between the two groups was assessed with unpaired t test.

(E) Silver-stained gels were used to assess the distribution of MyHC isoforms in RR (n = 7) and XX (n = 7) individuals. Right part shows a representative example of the distribution of MyHC in RR and XX individuals. The total staining of the three MyHC bands was set to 1 in each subject. Statistical difference between the two groups was tested with unpaired t test.

(F) Mean-centered sigma-normalized heatmap of differentially expressed proteins (p < 0.05).

(G) Volcano plot of all identified proteins (n = 601) expressed as fold-change (FC) in XX compared to RR individuals. Differentially abundant proteins are indicated in red. ...View More

Wyckelsma, V. L., Venckunas, T., Houweling, P. J., Schlittler, M., Lauschke, V. M., Tiong, C. F., ... & Westerblad, H. (2021). Loss of α-actinin-3 during human evolution provides superior cold resilience and muscle heat generation. The American Journal of Human Genetics, 108(3), 446-457.

Rabbit Anti-ACTN3 Recombinant Antibody (CBYC-A261) (CBMAB-A0995-YC) in IP

Endogenous interaction of Mib1 and α-Actn3 (Actn3) in GA muscle lysates. GA muscle lysates from 3-month-old WT mice were subjected to endogenous IP using anti-Mib1 and Actn3 antibody. Mouse and rabbit IgG were used as a nonspecific control. ...View More

Seo, J. Y., Kang, J. S., Kim, Y. L., Jo, Y. W., Kim, J. H., Hann, S. H., ... & Kong, Y. Y. (2021). Maintenance of type 2 glycolytic myofibers with age by Mib1-Actn3 axis. Nature Communications, 12(1), 1294.

Rabbit Anti-ACTN3 Recombinant Antibody (CBYC-A261) (CBMAB-A0995-YC) in WB

SDS-Page of age-matched controls and patient P1 muscle protein extracts revealed with antibodies against alpha-actinin-3 and beta-tubulin showed strong reduction of alpha-actinin-3 in the patient's biopsy. ...View More

Lornage, X., Romero, N. B., Grosgogeat, C. A., Malfatti, E., Donkervoort, S., Marchetti, M. M., ... & Laporte, J. (2019). ACTN2 mutations cause "multiple structured core disease"(MsCD). Acta neuropathologica, 137, 501-519.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

V2-179648

Application

WB, IHC, ICC, IHC-F

Immunogen

A synthetic peptide corresponding to residues on the N-terminus of human alpha-actinin 3

Host Species

Rabbit

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:5,000-1:10,000
IHC-P	1:100-1:250
FC	1:30

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

pH 7.4, 0.01% sodium azide, 50% glycerol, 0.05% BSA

Buffer

PBS, pH7.2, 0.05% BSA, 40% Glycerol

Preservative

0.01% sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Actinin Alpha 3 (Gene/Pseudogene)

Introduction

ACTN3 is a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin fi

Entrez Gene ID

Human	89
Mouse	11474
Rat	171009

UniProt ID

Human	Q08043
Mouse	O88990
Rat	Q8R4I6

Alternative Names

Actinin Alpha 3 (Gene/Pseudogene); F-Actin Cross-Linking Protein; Actinin, Alpha 3; Alpha-Actinin Skeletal Muscle Isoform 3; Alpha-Actinin Skeletal Muscle; Alpha-Actinin-3; ACTN3D;

Function

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

Biological Process

Focal adhesion assembly
Muscle filament sliding
Negative regulation of calcineurin-NFAT signaling cascade
Negative regulation of cold-induced thermogenesis
Negative regulation of glycolytic process
Negative regulation of oxidative phosphorylation
Positive regulation of glucose catabolic process to lactate via pyruvate
Positive regulation of skeletal muscle tissue growth
Regulation of aerobic respiration
Regulation of apoptotic process
Regulation of the force of skeletal muscle contraction
Response to denervation involved in regulation of muscle adaptation
Skeletal muscle atrophy
Transition between fast and slow fiber

Cellular Location

Actin filament; Cytosol; Extracellular exosome; Focal adhesion

Seto, J. T., Roeszler, K. N., Meehan, L. R., Wood, H. D., Tiong, C., Bek, L., ... & North, K. N. (2021). ACTN3 genotype influences skeletal muscle mass regulation and response to dexamethasone. Science Advances, 7(27), eabg0088.

Habibullayevna, B. G., Sobitjanovna, A. S., Makhmusovna, M., & Yusubjanovna, Z. (2020). Optimization Of Molecular-Genetic Methods For The Determination Of Resistance Markers Using Genotyping Of Actn3 And Ace Genes. European Journal of Molecular & Clinical Medicine, 7(10), 67-74.

Shumna, T. Y., Voznyi, O. V., Zinchenko, T. P., & Kamyshnyi, O. M. (2020). Frequency analysis of the ACTN3 gene polymorphism in children with isolated or combined orthodontic pathology with allergic rhinitis. Journal of Education, Health and Sport, 10(7), 41-55.

Massidda, M., Voisin, S., Culigioni, C., Piras, F., Cugia, P., Yan, X., ... & Calò, C. M. (2019). ACTN3 R577X polymorphism is associated with the incidence and severity of injuries in professional football players. Clinical Journal of Sport Medicine, 29(1), 57-61.

Ropka-Molik, K., Stefaniuk-Szmukier, M., Musiał, A. D., Piórkowska, K., & Szmatoła, T. (2019). Sequence analysis and expression profiling of the equine ACTN3 gene during exercise in Arabian horses. Gene, 685, 149-155.

Del Coso, J., Hiam, D., Houweling, P., Pérez, L. M., Eynon, N., & Lucia, A. (2019). More than a ‘speed gene’: ACTN3 R577X genotype, trainability, muscle damage, and the risk for injuries. European journal of applied physiology, 119(1), 49-60.

Ma, T., Lu, D., Zhu, Y. S., Chu, X. F., Wang, Y., Shi, G. P., ... & Wang, X. F. (2018). ACTN3 genotype and physical function and frailty in an elderly Chinese population: the Rugao Longevity and Ageing Study. Age and ageing, 47(3), 416-422.

Eroğlu, O., Zileli, R., Nalbant, M. A., & Ulucan, K. (2018). Prevalence of alpha actinin-3 gene (ACTN3) R577X and angiotensin converting enzyme (ACE) insertion/deletion gene polymorphisms in national and amateur Turkish athletes. Cellular and Molecular Biology, 64(5), 24-28.

Houweling, P. J., Papadimitriou, I. D., Seto, J. T., Pérez, L. M., Coso, J. D., North, K. N., ... & Eynon, N. (2018). Is evolutionary loss our gain? The role of ACTN3 p. Arg577Ter (R577X) genotype in athletic performance, ageing, and disease. Human mutation, 39(12), 1774-1787.

Garton, F. C., Houweling, P. J., Vukcevic, D., Meehan, L. R., Lee, F. X., Lek, M., ... & North, K. N. (2018). The effect of ACTN3 gene doping on skeletal muscle performance. The American Journal of Human Genetics, 102(5), 845-857.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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